Optimization and Validation of an LC-FLD Method for Biotin in Infant Formula, Infant Cereals, Cocoa-Malt Beverages, and Clinical Nutrition Products

• Published in: Journal of AOAC International Vol. 93; no. 5; pp. 1494 - 1502
• Main Authors: CAMPOS GIMENEZ, Esther, TRISCONI, Marie-Jose, MLINC, Tamara, ANDRIEUX, Pierre
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01.09.2010; Oxford University Press
• Subjects: Beverages - analysis; Biological and medical sciences; Biotin; Biotin - analysis; Cacao - chemistry; Cereal and baking product industries; Chromatography; Chromatography, High Pressure Liquid - methods; Edible Grain - chemistry; Feeding. Feeding behavior; Fluorescence; Food industries; Fundamental and applied biological sciences. Psychology; Grain; Health aspects; Humans; Infant; Infant Formula - chemistry; Methods; Nutrition; Papain; Physiological aspects; Reproducibility of Results; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Switzerland; Validation; Cocoa beverage; Nutrition; Vitamin; Malt; Cereal; Biotin; Liquid chromatography; Method; Infant formula; Optimization

A fast and simple chromatographic method to determine biotin in foods is presented. Biotin is extracted using papain (60 degrees C, 1 h). After pH adjustment and filtration, biotin is determined by LC with fluorescence detection using postcolumn reagent avidin-FITC (avidin labeled with fluorescein i...