Optimization and Validation of an LC-FLD Method for Biotin in Infant Formula, Infant Cereals, Cocoa-Malt Beverages, and Clinical Nutrition Products

• Published in: Journal of AOAC International Vol. 93; no. 5; pp. 1494 - 1502
• Main Authors: CAMPOS GIMENEZ, Esther, TRISCONI, Marie-Jose, MLINC, Tamara, ANDRIEUX, Pierre
• Format: Journal Article
• Language: English
• Published: Gaithersburg, MD AOAC International 01.09.2010; Oxford University Press
• Subjects: Beverages - analysis; Biological and medical sciences; Biotin; Biotin - analysis; Cacao - chemistry; Cereal and baking product industries; Chromatography; Chromatography, High Pressure Liquid - methods; Edible Grain - chemistry; Feeding. Feeding behavior; Fluorescence; Food industries; Fundamental and applied biological sciences. Psychology; Grain; Health aspects; Humans; Infant; Infant Formula - chemistry; Methods; Nutrition; Papain; Physiological aspects; Reproducibility of Results; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Switzerland; Validation; Cocoa beverage; Nutrition; Vitamin; Malt; Cereal; Biotin; Liquid chromatography; Method; Infant formula; Optimization
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoac/93.5.1494

A fast and simple chromatographic method to determine biotin in foods is presented. Biotin is extracted using papain (60 degrees C, 1 h). After pH adjustment and filtration, biotin is determined by LC with fluorescence detection using postcolumn reagent avidin-FITC (avidin labeled with fluorescein isothiocyanate). The method has been validated in a large range of products: milk- and soy-based infant formulas, cereals, cocoa-malt beverages, and clinical nutrition products. The method showed recovery rates of 98.1 +/- 5.7% (average +/- SD) in a large range of concentrations. Biotin concentrations determined in infant formula standard reference materials 1846 and 1849 were in agreement with reference values. RSD of repeatability (RSDr) varied from 2.0 to 4.5%, and intermediate reproducibility (RSD(iR)) from 5.8 to 9.4%. LOD and LOQ were 3.0 and 5.0 microg/100 g, respectively. The proposed method is suitable for routine analysis of biotin in fortified foods (infant formulas, infant cereals, cocoa-malt beverages, and clinical nutrition products). It can be used as a faster, more selective, and precise alternative to the classical microbiological determination, and is easily transferable among laboratories.