Structures of filum terminale and characteristics of ependymal cells of its central canal in rats

• Published in: Brain research Vol. 1707; pp. 208 - 215
• Main Authors: Nakano, Norihiko, Kanekiyo, Kenji, Yamada, Yoshihiro, Tamachi, Masahiro, Suzuki, Yoshihisa, Fukushima, Masatoshi, Saito, Fukuki, Abe, Seiya, Tsukagoshi, Chihiro, Miyamoto, Chimi, Ide, Chizuka
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.03.2019
• Subjects: Cauda equine; Central canal; Conus medullaris; Ependymal cell; Filum terminale; Transplant; Ependymal cell; Central canal; Conus medullaris; Filum terminale; Cauda equine; Transplant
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2018.11.038

•The filum terminale contains ependymal cells lining the central canal.•The conus medullaris should not be included into the filum terminale.•The rostral part of the filum terminale is included within the cauda equina.•This part (filum terminale internum) is exposed to the cerebrospinal fluid.•Ependymal cells of this part can be used as a transplant for the spinal cord injury. The filum terminale (FT) is a potential source of ependymal cells for transplantation. The present study was performed to clarify the characteristics of ependymal cells of the central canal (CC) of the FT in rats. The FT was a thin strand continuous with the conus medullaris (CM), a caudal end of the main spinal cord, situated at the L3-4 level in adult rats. The border between the CM and FT was not visible, but could be defined as the site where the strand was as thin as its more caudal segment. While the CM contained an appreciable amount of white and grey matter associated with the CC at its center, the FT had no or only a negligible amount of such spinal cord parenchymal tissue. The FT was tracked ca. 4 cm from the site defined above to the level of S4-5 in adult rats. The rostral part of the FT (FTI) included within the cauda equina is exposed to cerebrospinal fluid, whereas the more caudal part (FTE) was surrounded by a dense layer of connective tissue. Almost all ependymal cells were immunostained for Sox2, Sox9, FoxJ1, and CD133, generally recognized immunochemical markers for ependymal cells of the CC in the spinal cord. Ependymal cells of the CC of FT exhibited almost the same structural and immunohistochemical characteristics as those of the CC of the main spinal cord. Ependymal cells of FTI covered by a thin layer of connective tissue are considered appropriate for transplantation.