Recognition of Remote Mismatches by DNA Polymerases

Not only the first! We show that DNA polymerases inspect mismatches within the primer–template complex up to four positions distal to the catalytic center, as shown. Insights into enzyme transitions were drawn by chemical probes and extended to direct analysis of single nucleotide variations through...

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Bibliographic Details
Published inChembiochem : a European journal of chemical biology Vol. 5; no. 11; pp. 1585 - 1588
Main Authors Strerath, Michael, Gaster, Jens, Marx, Andreas
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 05.11.2004
WILEY-VCH Verlag
WILEY‐VCH Verlag
Subjects
Base Pair Mismatch
DNA
DNA - chemistry
DNA - genetics
DNA polymerase
DNA replication
DNA-Directed DNA Polymerase - chemistry
oligonucleotides
polymerase chain reaction
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Summary:Not only the first! We show that DNA polymerases inspect mismatches within the primer–template complex up to four positions distal to the catalytic center, as shown. Insights into enzyme transitions were drawn by chemical probes and extended to direct analysis of single nucleotide variations through allele‐specific PCR.
Bibliography:http://dx.doi.org/10.1002/cbic.200400107
ArticleID:CBIC200400107
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ISSN:1439-4227
1439-7633
1439-4227
DOI:10.1002/cbic.200400107