PTH induces modification of transductive events in otosclerotic bone cell cultures

We studied the effect of PTH (10-100 nM) on transductive mechanisms (adenylate cyclase activity, Ca2+ metabolism, IP3 levels) in cell cultures derived from normal and otosclerotic human bone fragments. The cultured cells were osteoblast-like but with calcitonin-receptors still present and with PTH r...

Full description

Saved in:
Bibliographic Details
Published inCell biochemistry and function Vol. 11; no. 4; p. 257
Main Authors Fanó, G, Venti-Donti, G, Belia, S, Paludetti, G, Antonica, A, Donti, E, Maurizi, M
Format Journal Article
LanguageEnglish
Published England 01.12.1993
Subjects
Adenylyl Cyclases - analysis
Bone and Bones - drug effects
Bone and Bones - metabolism
Calcium - metabolism
Cells, Cultured
Humans
Inositol Phosphates - analysis
Otosclerosis - metabolism
Parathyroid Hormone - pharmacology
Signal Transduction - drug effects
Online AccessGet more information

Cover

More Information
Summary:We studied the effect of PTH (10-100 nM) on transductive mechanisms (adenylate cyclase activity, Ca2+ metabolism, IP3 levels) in cell cultures derived from normal and otosclerotic human bone fragments. The cultured cells were osteoblast-like but with calcitonin-receptors still present and with PTH receptors coupled with the adenylate cyclase system. The results showed that PTH activated adenylate cyclase and increased the intracellular Ca2+ levels with qualitative and quantitative differences between the two cellular populations. In particular, otosclerotic cells responded less to hormone stimulation, which is in accord with the current hypothesis of a desensitization of the receptor/enzyme complex associated with the pathological status.
ISSN:0263-6484
1099-0844
DOI:10.1002/cbf.290110406