l-Arginine and melatonin interaction in rat intestinal ischemia-reperfusion

• Published in: Fundamental & clinical pharmacology Vol. 19; no. 5; pp. 533 - 535
• Main Authors: Arslan, S. Oktay, Gelir, Ethem, Sayan, Hale, Haktan Ozacmak, V.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.10.2005; Blackwell
• Subjects: Acetylcholine - pharmacology; Animals; Anticonvulsants - pharmacology; Arginine - pharmacology; Biological and medical sciences; contractile activity; Drug Synergism; Ileum - blood supply; Ileum - drug effects; Ileum - physiopathology; interaction; ischemia and reperfusion; l-arginine; Male; Medical sciences; melatonin; Melatonin - pharmacology; Mesentery - blood supply; Mesentery - drug effects; Mesentery - physiopathology; Muscle Contraction - drug effects; Muscle, Smooth - blood supply; Muscle, Smooth - drug effects; Muscle, Smooth - physiopathology; Pharmacology. Drug treatments; Rats; Rats, Wistar; Reperfusion Injury - physiopathology; Vasodilator Agents - pharmacology; Melatonin; Rat; Interaction; Ischemia reperfusion; Rodentia; interaction. ischemia and reperfusion; L-arginine; Biological activity; Vertebrata; Mammalia; Arginine; Aminoacid; Animal; Intestinal ischemia; contractile activity; Pineal hormone
• ISSN: 0767-3981; 1472-8206
• DOI: 10.1111/j.1472-8206.2005.00365.x

We investigated the combinative effects of l‐arginine and melatonin on the contractile responses of terminal ileum after the intestinal ischemia–reperfusion (I/R), in vivo. Male rats were subjected to mesenteric ischemia (30 min) followed by reperfusion (180 min). We have observed a dramatic decrease in spontaneous basal activity and Ach‐induced contractile response. Our data clearly showed that the contractility decrease was ameliorated by l‐arginine but not by l‐NAME. Melatonin has reversed the inhibition of contractility caused by I/R injury in part. We did not observe an augmentation in the contractility of ileum when we use melatonin and l‐arginine in combination, in fact, melatonin decreased the protective effect of l‐arginine in intestinal I/R injury.