Catalytic potential of a nitrocellulose membrane-immobilized lipase in aqueous and organic media

• Published in: Journal of applied polymer science Vol. 124; no. S1; pp. E37 - E44
• Main Authors: Kumar, Ashok, Kanwar, Shamsher S.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 25.06.2012; Wiley Subscription Services, Inc
• Subjects: Cellulose esters; Enzymes; Esters; formalin; immobilization; Lipase; Liquid chromatography; Materials science; Mathematical models; Membranes; Nitrocellulose; nitrocellulose membrane; octyl ferulate; Polymers
• ISSN: 0021-8995; 1097-4628
• DOI: 10.1002/app.35434

Artificial membranes are ideal supports for enzyme immobilization and biocatalysis. The binding of a commercial lipase, Lipolase T20 on to nitrocellulose (NC) membrane was achieved by physical adsorption followed by cross‐linking with formalin (2%). The NC‐immobilized lipase was characterized for its catalytic activity and operational stability, using p‐nitrophenyl palmitate (p‐NPP) hydrolysis as a model reaction system. Functionally membrane bound lipase was more stable than free lipase (55°C) at higher temperature (65 and 75°C) and at pH 9.0 to 9.5. The hydrolytic properties of NC‐bound lipase were studied consecutively as a function of physicochemical parameters. The NC‐bound lipase was highly hydrolytic toward relatively longer C‐chain length esters. This indicated a preferential selectivity of NC‐bound lipase toward the p‐NPP with an activity of 6.03 + 0.30 IU/cm2. The nonionic detergents like Tween‐20, 40, and 60 promoted the hydrolytic activity of membrane‐bound lipase, while Tween‐80 prompted a decline in the activity of NC‐membrane bound lipase. Among various salt ions, Ca2+, Al3+, Zn2+, and Cu2+ showed a stimulatory effect while Fe2+, NH4+, Cd2+, and Zn2+ antagonized the hydrolytic activity of the bound lipase. The immobilized lipase was studied for its reusability and was found to retain >50% of hydrolytic activity after fifth repetitive cycle. In DMSO, the synthesis of octyl ferulate at 55°C under shaking (150 rpm) using ferulic acid (75 mM) and 1‐octanol (100 mM) was achieved with a yield of ˜61 mM of ester (1‐octyl ferulate) as analyzed by gas liquid chromatography. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012