Zona pellucida removal resulted in a successful live birth: Report on a case with recurrent implantation failure due to embryonic bacteria contamination

• Published in: Clinical case reports Vol. 12; no. 2; pp. e8483 - n/a
• Main Authors: Dang, Son Truong, Tran, Huy Phuong, Ho, Tuong Nguyen, Ly, Loc Thai, Hoang, Tuyet Thi‐Diem, Huynh, Trang Nguyen‐Khanh, Do, Anh Tuan, Nguyen, Thuan Duc, Dao, Phuong Thi
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.02.2024; John Wiley and Sons Inc; Wiley
• Subjects: Bacteria; Body mass index; Case Report; Case reports; contaminated embryo; embryo transfer; Embryos; Endometrium; Genetic testing; In vitro fertilization; IVF; Lasers; Microorganisms; Obstetrics/Gynecology; recurrent implantation failure; Sperm; zona pellucida removal; Japan; recurrent implantation failure; IVF; contaminated embryo; zona pellucida removal; embryo transfer
• ISSN: 2050-0904; 2050-0904
• DOI: 10.1002/ccr3.8483

Key clinical message In in vitro fertilization (IVF), laser offers several advantages. In this study, we employed laser to eliminate the zona pellucida of a contaminated embryo. This approach helps to rescue embryo with bacterial contamination, and improve embryo‐endometrium interaction. To present a case report on the removal of a contaminated zona pellucida from an embryo of patient with a history of recurrent implantation failure (RIF), which was followed by a successful live birth. We present the case of a 34‐year‐old patient with a history of 3 years of infertility who underwent in vitro fertilization. During the culture process, the embryos became contaminated, leading to three failed implantations. Despite the aneuploidy of the embryo and the implementation of a washing technique, the contamination persisted. In the final attempt, the contaminated zona pellucida was successfully removed using laser, followed by embryo transfer, resulting in a live birth. We provided detailed clinical information, including patient demographics, infertility history, ovarian response, evidence of bacterial contamination, embryo development, treatment protocols, and outcomes. Laser excision of the zona pellucida is a safe and effective method for addressing bacterial infection in embryos.