Fidelity of peripheral blood for monitoring genomics and tumor immune‐microenvironment in myelodysplastic syndromes

The aim of this study is to investigate whether the peripheral blood (PB) can serve as a surrogate immune‐microenvironment to bone marrow for genetic and immune monitoring in myelodysplastic syndrome (MDS). We compared the composition of T cell subsets and somatic mutation burden in 36 pairs of PB a...

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Published inEJHaem Vol. 1; no. 2; pp. 552 - 557
Main Authors Lee, Sung‐Eun, Wang, Feng, Trujillo‐Ocampo, Abel, Ruiz‐Vasquez, Wilfredo, Cho, Hyun‐Woo, Takahashi, Koichi, Molldrem, Jeffrey J., Futreal, Andrew, Garcia‐Manero, Guillermo, Im, Jin S.
Format Journal Article
LanguageEnglish
Published United States John Wiley and Sons Inc 01.11.2020
Wiley
Subjects
bone marrow aspirate
genomics
myelodysplastic syndrome
peripheral blood
Short Report
Short Reports
tumor immune‐microenvironment
bone marrow aspirate
genomics
tumor immune‐microenvironment
peripheral blood
myelodysplastic syndrome
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Summary:The aim of this study is to investigate whether the peripheral blood (PB) can serve as a surrogate immune‐microenvironment to bone marrow for genetic and immune monitoring in myelodysplastic syndrome (MDS). We compared the composition of T cell subsets and somatic mutation burden in 36 pairs of PB and matching bone marrow aspirate (BMA) using multi‐parameter flow cytometry and NGS‐based targeted sequencing analysis, respectively. Our immune‐subset and NGS‐based mutation analysis of BMA showed significant concordance with those of PB in MDS. Therefore, PB can provide easily accessible tumor immune‐microenvironment for monitoring in the immune and genetic landscapes for MDS patients.
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ISSN:2688-6146
2688-6146
DOI:10.1002/jha2.112