Generation of Pseudocontact Shifts in Protein NMR Spectra with a Genetically Encoded Cobalt(II)-Binding Amino Acid

Playing tag: Incorporation of the genetically encoded amino acid bipyridylalanine into a protein creates a site‐specific binding site for CoII. Pronounced pseudocontact shifts generated by the CoII ion in the NMR spectra delivers powerful long‐range structure information for the facile analysis of p...

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Published inAngewandte Chemie (International ed.) Vol. 50; no. 3; pp. 692 - 694
Main Authors Nguyen, Thi Hoang Duong, Ozawa, Kiyoshi, Stanton-Cook, Mitchell, Barrow, Russell, Huber, Thomas, Otting, Gottfried
Format Journal Article
LanguageEnglish
Published Weinheim Wiley-VCH Verlag 17.01.2011
WILEY-VCH Verlag
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
2,2'-Dipyridyl - chemistry
Alanine - chemistry
Amino acids
Amino Acids - chemistry
Binding Sites
cell-free protein synthesis
Cobalt
Cobalt - chemistry
Magnetic Resonance Spectroscopy
NMR spectroscopy
Protein Binding
protein modifications
Protein Structure, Tertiary
protein structures
Proteins - chemistry
pseudocontact shifts
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Summary:Playing tag: Incorporation of the genetically encoded amino acid bipyridylalanine into a protein creates a site‐specific binding site for CoII. Pronounced pseudocontact shifts generated by the CoII ion in the NMR spectra delivers powerful long‐range structure information for the facile analysis of proteins as well as protein–protein and protein–ligand complexes (see picture for a representation of the psuedocontact shift in the protein).
Bibliography:http://dx.doi.org/10.1002/anie.201005672
ark:/67375/WNG-V4QKF395-Q
We thank Prof. P. G. Schultz for in vivo expression systems of suppressor tRNA and MjTyrRS, Dr. Siew Pheng Lim for the expression system of WNVpro, Dr. Ruhu Qi for help with protein expression, and Dr. Hiromasa Yagi and Laura de la Cruz for help with NMR spectroscopy. Support of this research by the Australian Research Council included fellowships to K.O. and T.H.
ArticleID:ANIE201005672
istex:B5B5A342B5596025E96F8939E383634C60BED1D4
Australian Research Council
We thank Prof. P. G. Schultz for in vivo expression systems of suppressor tRNA and
Mj
TyrRS, Dr. Siew Pheng Lim for the expression system of WNVpro, Dr. Ruhu Qi for help with protein expression, and Dr. Hiromasa Yagi and Laura de la Cruz for help with NMR spectroscopy. Support of this research by the Australian Research Council included fellowships to K.O. and T.H.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201005672