The effects of bisphenol A and bisphenol S on adipokine expression and glucose metabolism in human adipose tissue

•BPA reduces expression of proinflammatory adipokines in human adipose tissue.•BPS has no effect on proinflammatory gene expression in human adipose tissue.•BPA reduces glucose uptake in human subcutaneous adipocytes.•BPA does not alter gene expression and protein levels in the insulin signalling pa...

Full description

Saved in:
Bibliographic Details
Published inToxicology (Amsterdam) Vol. 445; p. 152600
Main Authors Ahmed, Fozia, Sarsenbayeva, Assel, Katsogiannos, Petros, Aguer, Céline, Pereira, Maria J
Format Journal Article
LanguageEnglish
Published Ireland Elsevier B.V 01.12.2020
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:•BPA reduces expression of proinflammatory adipokines in human adipose tissue.•BPS has no effect on proinflammatory gene expression in human adipose tissue.•BPA reduces glucose uptake in human subcutaneous adipocytes.•BPA does not alter gene expression and protein levels in the insulin signalling pathway. The environmental endocrine disruptors, bisphenol A (BPA) and bisphenol S (BPS) are associated with the development of type 2 diabetes. We aim to study the effects of BPA or BPS exposure on adipokine expression in human adipose tissue and on adipocyte glucose uptake. Human subcutaneous adipose tissue was treated for 24 or 72 h with environmentally-relevant and supraphysiological concentrations of BPA or BPS (1–104 nM). Following exposure, gene expression of proinflammatory cytokines, adipokines, and estrogen receptors was measured in adipose tissue. Glucose uptake and the insulin signalling pathway were analyzed in isolated adipocytes following adipose tissue culture with BPA for 24 h. Adipose tissue treated with BPA for 24 h had reduced expression of the proinflammatory genes (IL6, IL1B, TNFA) and adipokines (ADIPOQ, FABP4). BPA and BPS had no effect on the expression of other proinflammatory genes (IL33), adipokines (LEP), or receptors (ESR1, ESR2) after 72-h exposure. Adipose tissue treated with environmentally-relevant concentrations of BPA for 24 h had reduced insulin-stimulated glucose uptake, without altered gene and protein levels of key insulin signalling pathway markers. We found that human adipose tissue treated with environmentally-relevant concentrations of BPA for 24 h, but not BPS, reduced expression of proinflammatory genes and adipokines. Furthermore, BPA reduced glucose uptake in adipocytes independently of insulin signalling. Such mechanisms can contribute to the development of insulin resistance associated with BPA exposure.
ISSN:0300-483X
1879-3185
1879-3185
DOI:10.1016/j.tox.2020.152600