Antimicrobial properties of the chelating agent EDTA on streptococcal bovine mastitis isolates

• Published in: Journal of food protection Vol. 69; no. 6; pp. 1460 - 1462
• Main Authors: Reidmiller, J.S, Smith, W.L, Sawyer, M.M, Osburn, B.I, Stott, J.L, Cullor, J.S
• Format: Journal Article
• Language: English
• Published: Des Moines, IA International Association of Milk, Food and Environmental Sanitarians 01.06.2006
• Subjects: Animal productions; Animals; Anti-Bacterial Agents - pharmacology; antibacterial properties; bacterial infections; Biological and medical sciences; bovine mastitis; Cattle; Chelating Agents; Colony Count, Microbial; Dose-Response Relationship, Drug; Edetic Acid - pharmacology; EDTA (chelating agent); Female; Food Additives - pharmacology; Food Contamination; Food industries; Food Microbiology; food preservatives; Fundamental and applied biological sciences. Psychology; in vitro culture; Mastitis, Bovine - drug therapy; milk; Milk - microbiology; minimum inhibitory concentration; Streptococcus; Streptococcus - drug effects; Streptococcus agalactiae; Streptococcus agalactiae - drug effects; Streptococcus uberis; Terrestrial animal productions; Vertebrates; veterinary medicine; Mammary gland diseases; Vertebrata; Mastitis; Mammalia; Bovine; Chelating agent; Isolate; Artiodactyla; EDTA; Properties; Ungulata; Antimicrobial agent
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028X-69.6.1460

To determine the efficacy of the chelating agent EDTA on microbial growth, separate cultures of two streptococcal bovine mastitis isolates, Streptococcus agalactiae and Streptococcus uberis, were exposed to known concentrations of EDTA. Bacterial cultures of 10(8) CFU/ml were exposed to concentrations of EDTA ranging from 30 to 100 mM in an in-vitro-milk environment. Multiple replications of cultures exposed to EDTA were plated during a two-hour time course. A concentration of 100 mM EDTA resulted in a 90% reduction of S. agalactiae and a 99% reduction of S. uberis. Under these experimental conditions, EDTA treatments in cultures of both isolates exhibited from 1 to 2 log reductions suggesting that EDTA is a potentially effective antimicrobial against streptococcal isolates implicated in causing bovine mastitis.