Parathyroid hormone-related protein (1-37) induces cAMP response in human osteoblast-like cells

• Published in: Calcified tissue international Vol. 62; no. 3; pp. 250 - 254
• Main Authors: Tsai, J A, Bucht, E, Stark, A, Sjöstedt, U, Törring, O
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.03.1998
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Alkaline Phosphatase - metabolism; Animals; Calcitriol - pharmacology; CHO Cells - drug effects; CHO Cells - metabolism; Cricetinae; Cyclic AMP - metabolism; Dose-Response Relationship, Drug; Female; Humans; Male; Medicin och hälsovetenskap; Middle Aged; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteocalcin - metabolism; Parathyroid Hormone-Related Protein; Proteins - pharmacology; Rats; Teriparatide - pharmacology; Tumor Cells, Cultured
• ISSN: 0171-967X; 1432-0827
• DOI: 10.1007/s002239900425

During recent years parathyroid hormone-related protein (PTHrP) research has been focused on the physiological functions of different fragments of the PTHrP molecule. Here we demonstrate that PTHrP (1-37) induced cyclic adenosine monophosphate (cAMP) response in primary human osteoblast-like cells, which were well characterized by the presence of alkaline phosphatase activity and osteocalcin production after stimulation with 1,25-dihydroxyvitamin D3. However, there was no cAMP response to PTHrP (58-77). Furthermore, the response to PTHrP (1-37) was dose dependent, with a significant increase at 1 nM. The presence of PTHrP (1-37)-induced cAMP response in human osteoblast-like cells implies that aminoterminal PTHrP fragments may exert important functions in the bone.