A functional neo-centromere formed through activation of a latent human centromere and consisting of non-alpha-satellite DNA

• Published in: Nature genetics Vol. 16; no. 2; pp. 144 - 153
• Main Authors: Sart, Desirée du, Saffery, Richard, Barry, Alyssa E, Kalitsis, Paul, Cancilla, Michael R, Tainton, Kellie M, Martyn, John, Choo, K. H. Andy, Mao, Jen-i, Earle, Elizabeth
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group 01.06.1997
• Subjects: Binding Sites, Antibody; Biological and medical sciences; Centromere; Chromatin. Chromosome; Chromosomes, Artificial, Yeast; Chromosomes, Human, Pair 10; DNA, Satellite - genetics; DNA, Satellite - metabolism; Fundamental and applied biological sciences. Psychology; Genetic Markers; Humans; Hybrid Cells; In Situ Hybridization, Fluorescence; Molecular and cellular biology; Molecular genetics; Human; Centromere; Contig; Antigenic determinant; Antibody; Activation; Chromosome C10; Artificial chromosome
• ISSN: 1061-4036; 1546-1718
• DOI: 10.1038/ng0697-144

We recently described a human marker chromosome containing a functional neo-centromere that binds anti-centromere antibodies, but is devoid of centromeric alpha-satellite repeats and derived from a hitherto non-centromeric region of chromosome 10q25. Chromosome walking using cloned single-copy DNA from this region enabled us to identify the antibody-binding domain of this centromere. Extensive restriction mapping indicates that this domain has an identical genomic organization to the corresponding normal chromosomal region, suggesting a mechanism for the origin of this centromere through the activation of a latent centromere that exists within 10q25.