Subinhibitory Concentrations of Prim-O-Glucosylcimifugin Decrease the Expression of Alpha-Hemolysin in Staphylococcus aureus (USA300)

Staphylococcus aureus (S. aureus), an important opportunistic pathogen in human and animal, causes a series of diseases in the impairing of immunity of host and even then death. Alpha-hemolysin (Hla), a primary virulence factor, plays a major role in the pathogenic progress of S. aureus, especially...

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Published inEvidence-based complementary and alternative medicine Vol. 2018; no. 2018; pp. 1 - 8
Main Authors Tang, Li, Yin, Lizi, Weimin, Lai, Huang, Xiaoli, Geng, Yi, Fang, Jing, Wang, Kaiyu, Jiaqiang, Deng, Ruixue, Yang, Ou, Yangping, Chen, Defang
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 01.01.2018
Hindawi
Hindawi Limited
Subjects
Antibiotics
Antimicrobial agents
Bacteria
Bacterial infections
Chemotherapy
Epidemics
Gene expression
Herbal medicine
Histocompatibility antigen HLA
Infectious diseases
Medical research
Opportunist infection
Pneumonia
Polymerase chain reaction
Staphylococcus aureus
Staphylococcus infections
Streptococcus infections
Transcription
Virulence factors
Western blotting
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Summary:Staphylococcus aureus (S. aureus), an important opportunistic pathogen in human and animal, causes a series of diseases in the impairing of immunity of host and even then death. Alpha-hemolysin (Hla), a primary virulence factor, plays a major role in the pathogenic progress of S. aureus, especially in pneumonia. Prim-O-glucosylcimifugin (POG), a nature chromone compound, is an active ingredient in many Chinese Medicines. In this study, POG investigated the inhibitory effect of the secretion of Hla in S. aureus strain USA300 at the subinhibitory concentrations. The hemolysis assays and western blotting assays showed that POG can decrease the production of Hla in the USA300 growth cell cultures in a dose-dependent manner. The results of RT-PCR revealed that reduction of Hla was related to inhibit the transcription of hla and RNAIII. In the cells experiment, POG was proved to protect A549 cells from Hla-medicated injury. In conclusion, POG was shown the capacity of decreased the production of S. aureus Hla. POG can be developed as a candidate agent to treat S. aureus infections against Hla.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Academic Editor: José L. Rios
ISSN:1741-427X
1741-4288
DOI:10.1155/2018/7579808