A Multiomic Analysis of Chicken Serum Revealed the Modulation of Host Factors Due to Campylobacter jejuni Colonization and In-Water Supplementation of Eugenol Nanoemulsion

• Published in: Animals (Basel) Vol. 13; no. 4; p. 559
• Main Authors: Wagle, Basanta R, Quach, Austin, Yeo, Seungjun, Assumpcao, Anna L F V, Arsi, Komala, Donoghue, Annie M, Jesudhasan, Palmy R R
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.02.2023; MDPI
• Subjects: Acetic acid; Biomarkers; Birds; broiler chicken; Campylobacter; Campylobacter jejuni; Campylobacteriosis; Chickens; Colonization; Communication; Creatinine; Eugenol; Food contamination; Foodborne diseases; foodborne pathogen; Foodborne pathogens; Gastroenteritis; High mobility group proteins; Lipid metabolism; Lipids; Metabolites; multiomic analysis; Nanoemulsions; phytochemicals; Poultry; Proteins; Technology; LCMS-based technology; foodborne pathogen; multiomic analysis; eugenol; phytochemicals; broiler chicken; Campylobacter
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani13040559

is a foodborne pathogen that causes campylobacteriosis globally, affecting ~95 million people worldwide. Most infections involve consuming and/or handling improperly cooked poultry meat. To better understand chicken host factors modulated by colonization, we explored a novel LCMS-based multiomic technology using three experimental groups: (1) negative control, (2) positive control, and (3) eugenol nanoemulsion (EGNE) treatment (supplemented with 0.125% EGNE in the water) of broiler chickens (n = 10 birds/group). Birds in groups two and three were challenged with on day 7, and serum samples were collected from all groups on day 14. Using this multiomic analysis, we identified 1216 analytes (275 compounds, seven inorganics, 407 lipids, and 527 proteins). The colonization of significantly upregulated CREG1, creatinine, and 3-[2-(3-Hydroxyphenyl) ethyl]-5-methoxyphenol and downregulated sphingosine, SP d18:1, high mobility group protein B3, phosphatidylcholines (PC) P-20:0_16:0, PC 11:0_26:1, and PC 13:0_26:2. We found that 5-hydroxyindole-3-acetic acid significantly increased with the EGNE treatment when compared to the positive and negative controls. Additionally, the treatment increased several metabolites when compared to the negative controls. In conclusion, this study revealed several potential targets to control in broiler chickens.