The Occurrence of Bactoprenol in the Mesosome and Plasma Membranes of Lactobacillus casei and Lactobacillus plantarum

Dunn Nutritional Laboratory, University of Cambridge and Medical Research Council, Cambridge ABSTRACT Summary: A bout one-third of the C 55 isoprenoid alcohol, bactoprenol, in Lactobacillus casei and L. plantarum cells was in the mesosomes and two-thirds in the plasma membrane. After removal of boun...

Full description

Saved in:
Bibliographic Details
Published inJournal of general microbiology Vol. 70; no. 1; pp. 87 - 98
Main Authors THORNE, KAREEN J. I, BARKER, D. C
Format Journal Article
LanguageEnglish
Published England Soc General Microbiol 01.04.1972
Subjects
Alcohols - analysis
Alcohols - biosynthesis
Carbon Isotopes
Cell Membrane - analysis
Cell Membrane - metabolism
Cell Wall - metabolism
Edetic Acid
Lactobacillus - analysis
Lactobacillus - metabolism
Lactobacillus casei - analysis
Lactobacillus casei - metabolism
Mevalonic Acid - metabolism
Microscopy, Electron
Muramidase
Protoplasts - analysis
Protoplasts - metabolism
Online AccessGet full text

Cover

More Information
Summary:Dunn Nutritional Laboratory, University of Cambridge and Medical Research Council, Cambridge ABSTRACT Summary: A bout one-third of the C 55 isoprenoid alcohol, bactoprenol, in Lactobacillus casei and L. plantarum cells was in the mesosomes and two-thirds in the plasma membrane. After removal of bound lysozyme from the plasma membrane, the concentration of bactoprenol per mg protein was the same in the mesosomes and in the plasma membrane. Pulse-labelling experiments and chasing with unlabelled precursor showed that bactoprenol is synthesized in both the plasma and mesosome membranes and that mesosomal bactoprenol is not a precursor of plasma membrane bactoprenol. These findings are discussed in relation to the involvement of bactoprenol in cell-wall synthesis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-1287
DOI:10.1099/00221287-70-1-87