Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction

• Published in: Journal of functional biomaterials Vol. 13; no. 2; p. 59
• Main Authors: Uemura, Laercio, Baggio Simeoni, Rossana, Bispo Machado Júnior, Paulo André, Gavazzoni Blume, Gustavo, Kremer Gamba, Luize, Sgarbossa Tonial, Murilo, Baggio Simeoni, Paulo Ricardo, Stadler Tasca Ribeiro, Victoria, Silvestre, Rodrigo, de Carvalho, Katherine Athayde Teixeira, Napimoga, Marcelo Henrique, Cesar Francisco, Júlio, Guarita-Souza, Luiz Cesar
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.05.2022; MDPI
• Subjects: 15d-PGJ2; Angiogenesis; Animal models; anti-inflammatory; Antibodies; Autografts; Bone marrow; bone marrow mononuclear cells; Bone marrow transplantation; Bone surgery; Connexin 43; Coronary vessels; Echocardiography; Electrical junctions; Flow cytometry; Gap junctions; Heart attacks; Inflammation; Injection; Laboratory animals; Leukocytes (mononuclear); Myocardial Infarction; Myocardium; Nanoparticles; Ostomy; rats; Statistical analysis; Stem cell transplantation; Stem cells; Surgery; Transplantation; United States > US; Germany; anti-inflammatory; bone marrow mononuclear cells; Myocardial Infarction; rats; 15d-PGJ2
• ISSN: 2079-4983; 2079-4983
• DOI: 10.3390/jfb13020059

To investigate the effect of transplantation of stem cells from the bone marrow mononuclear cells (BMMC) associated with 15d-PGJ2-loaded nanoparticles in a rat model of chronic MI. Chronic myocardial infarction (MI) was induced by the ligation of the left anterior descending artery in 40 male Wistar rats. After surgery, we transplanted bone marrow associated with 15d-PGJ2-loaded nanoparticle by intramyocardial injection (10 cells/per injection) seven days post-MI. Myocardial infarction was confirmed by echocardiography, and histological analyses of infarct morphology, gap junctions, and angiogenesis were obtained. Our results from immunohistochemical analyses demonstrated the presence of angiogenesis identified in the transplanted region and that there was significant expression of connexin-43 gap junctions, showing a more effective electrical and mechanical integration of the host myocardium. This study suggests that the application of nanoparticle technology in the prevention and treatment of MI is an emerging field and can be a strategy for cardiac repair.