Human endothelial cells display a rapid tensional stress increase in response to tumor necrosis factor-α

• Published in: PloS one Vol. 17; no. 6; p. e0270197
• Main Authors: Brandt, Matthias, Gerke, Volker, Betz, Timo
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 24.06.2022; Public Library of Science (PLoS)
• Subjects: Biology and Life Sciences; Blood; Blood vessels; Cytokines; Earth Sciences; Endothelial cells; Histamine; Histamines; Inflammation; Inflammatory response; Leukocyte migration; Leukocytes; Medicine and Health Sciences; Microscopy; Monolayers; Necrosis; Permeability; Phosphorylation; Physical Sciences; Stress analysis; Substrates; Thrombin; Traction; Traction force; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Umbilical vein
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0270197

Endothelial cells form the inner layer of blood vessels, making them the first barrier between the blood and interstitial tissues; thus endothelial cells play a crucial role in inflammation. In the inflammatory response, one important element is the pro-inflammatory cytokine tumor necrosis factor- α (TNF- α ). While other pro-inflammatory agents like thrombin and histamine induce acute but transient changes in endothelial cells, which have been well studied biologically as well as mechanically, TNF- α is primarily known for its sustained effects on permeability and leukocyte recruitment. These functions are associated with transcriptional changes that take place on the timescale of hours and days. Here, we investigated the early mechanical action of TNF- α and show that even just 4 min after TNF- α was added onto human umbilical vein endothelial cell monolayers, there was a striking rise in mechanical substrate traction force and internal monolayer tension. These traction forces act primarily at the boundary of the monolayer, as was to be expected. This increased internal monolayer tension may, in addition to TNF- α ’s other well-studied biochemical responses, provide a mechanical signal for the cells to prepare to recruit leukocytes.