Performance of Microflex LT Biotyper and VITEK MS for Routine Identification of Yeasts

• Published in: Annals of laboratory medicine Vol. 38; no. 5; pp. 487 - 489
• Main Authors: Byun, Jung-Hyun, Yu, A-Reum, Kim, Myung Sook, Lee, Kyungwon
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Laboratory Medicine 01.09.2018; 대한진단검사의학회
• Subjects: Area Under Curve; Candida - chemistry; Candida - cytology; Catheters - microbiology; Humans; Letter to the Editor; Mycoses - diagnosis; Mycoses - microbiology; ROC Curve; Saccharomycetales - chemistry; Saccharomycetales - isolation & purification; Specimen Handling; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Sputum - microbiology; 병리학
• ISSN: 2234-3806; 2234-3814; 2234-3814
• DOI: 10.3343/alm.2018.38.5.487

Dear Editor, The epidemiology of yeast infections is rapidly evolving, leading to the emergence of uncommon yeasts [1]. Rapid identification, followed by appropriate antimicrobial therapy, is associated with lower mortality [2]. Conventional phenotypic methods cannot differentiate certain yeast species accurately [3]. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been introduced in clinical microbiology to facilitate rapid yeast identification [3]. MALDI-TOF MS for yeast identification requires special preparation, similar to that for Mycobacterium species and gram-positive bacteria [4]. We compared the yeast identification capabilities of two MALDI-TOF systems—the Microflex LT Biotyper (Bruker Daltonics, Leipzig, Germany) and the VITEK MS (bioMerieux, Marcy-l’Étoile, France)—with respect to different sample preparation methods. KCI Citation Count: 0