Cell Wall Microdomains in the External Glands of Utricularia dichotoma Traps

• Published in: International journal of molecular sciences Vol. 25; no. 11; p. 6089
• Main Authors: Płachno, Bartosz J, Kapusta, Małgorzata, Stolarczyk, Piotr, Feldo, Marcin, Świątek, Piotr
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.06.2024; MDPI
• Subjects: Antibodies; Antigenic determinants; arabinogalactan proteins; bladderworts; carnivorous plants; cell wall; Cell Wall - metabolism; cell wall microdomains; Cells; cuticle; Electron microscopes; Electronics industry; Immunohistochemistry; Labeling; Lamiales - metabolism; Mucoproteins - metabolism; Plant Proteins - metabolism; Proteins; Viral antibodies; Poland; Japan; arabinogalactan proteins; Lentibulariaceae; glands; bladderworts; transfer cells; cuticle; trichomes; scanning transmission electron microscopy; cell wall; carnivorous plants; cell wall microdomains
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25116089

The genus (bladderworts) species are carnivorous plants that prey on invertebrates using traps with a high-speed suction mechanism. The outer trap surface is lined by dome-shaped glands responsible for secreting water in active traps. In terminal cells of these glands, the outer wall is differentiated into several layers, and even cell wall ingrowths are covered by new cell wall layers. Due to changes in the cell wall, these glands are excellent models for studying the specialization of cell walls (microdomains). The main aim of this study was to check if different cell wall layers have a different composition. Antibodies against arabinogalactan proteins (AGPs) were used, including JIM8, JIM13, JIM14, MAC207, and JIM4. The localization of the examined compounds was determined using immunohistochemistry techniques and immunogold labeling. Differences in composition were found between the primary cell wall and the cell secondary wall in terminal gland cells. The outermost layer of the cell wall of the terminal cell, which was cuticularized, was devoid of AGPs (JIM8, JIM14). In contrast, the secondary cell wall in terminal cells was rich in AGPs. AGPs localized with the JIM13, JIM8, and JIM14 epitopes occurred in wall ingrowths of pedestal cells. Our research supports the hypothesis of water secretion by the external glands.