Bacterial Purine Nucleoside Phosphorylases from Mesophilic and Thermophilic Sources: Characterization of Their Interaction with Natural Nucleosides and Modified Arabinofuranoside Analogues

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 9; p. 1069
• Main Authors: Bychek, Irina A, Zenchenko, Anastasia A, Kostromina, Maria A, Khisamov, Marat M, Solyev, Pavel N, Esipov, Roman S, Mikhailov, Sergey N, Varizhuk, Irina V
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.08.2024; MDPI
• Subjects: Adenosine; Bacteria - enzymology; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; biochemistry; Cell culture; Chromatography; E coli; Enzymatic synthesis; Enzymes; Glycerol; Kinetics; mesophilic enzymes; modified nucleoside analogues; Nelarabine; Nucleoside analogs; Nucleosides; Nucleosides - chemistry; Nucleosides - metabolism; Physicochemical properties; Potassium; purine nucleoside phosphorylases; Purine-Nucleoside Phosphorylase - chemistry; Purine-Nucleoside Phosphorylase - metabolism; Purines; Reagents; Solvents; Substrate Specificity; Temperature; thermophilic enzymes; Russia; United States > US; Germany; mesophilic enzymes; modified nucleoside analogues; thermophilic enzymes; purine nucleoside phosphorylases; biochemistry
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14091069

The enzymatic synthesis of nucleoside derivatives is an important alternative to multi-step chemical methods traditionally used for this purpose. Despite several undeniable advantages of the enzymatic approach, there are a number of factors limiting its application, such as the limited substrate specificity of enzymes, the need to work at fairly low concentrations, and the physicochemical properties of substrates-for example, low solubility. This research conducted by our group is dedicated to the advantages and limitations of using purine nucleoside phosphorylases (PNPs), the main enzymes for the metabolic reutilization of purines, in the synthesis of modified nucleoside analogues. In our work, the substrate specificity of PNP from various bacterial sources (mesophilic and thermophilic) was studied, and the effect of substrate, increased temperature, and the presence of organic solvents on the conversion rate was investigated.