Preparation of mesoporous silica thin films by photocalcination method and their adsorption abilities for various proteins

• Published in: Materials Science & Engineering C Vol. 40; pp. 42 - 48
• Main Authors: Kato, Katsuya, Nakamura, Hitomi, Yamauchi, Yoshihiro, Nakanishi, Kazuma, Tomita, Masahiro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2014
• Subjects: Adsorption; Antibody; Arrays; Binding; Biocatalysis; Biosensors; Cytochromes c - chemistry; Cytochromes c - metabolism; ELISA; Enzyme-Linked Immunosorbent Assay; Gels - chemistry; Humans; Immobilized Proteins - chemistry; Immobilized Proteins - metabolism; Immunoglobulin G - chemistry; Immunoglobulin G - metabolism; Mesoporous silica; Platforms; Polyethylene Glycols - chemical synthesis; Polyethylene Glycols - chemistry; Porosity; Propylene Glycols - chemical synthesis; Propylene Glycols - chemistry; Protein A; Silanes - chemistry; Silicon dioxide; Silicon Dioxide - chemistry; Staphylococcal Protein A - chemistry; Staphylococcal Protein A - metabolism; Thin film; Thin films; Ultraviolet Rays; Protein A; Antibody; Mesoporous silica; Thin film; ELISA
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2014.03.045

Mesoporous silica (MPS) thin film biosensor platforms were established. MPS thin films were prepared from tetraethoxysilane (TEOS) via using sol–gel and spin-coating methods using a poly-(ethylene oxide)-block-poly-(propylene oxide)-block-poly-(ethylene oxide) triblock polymer, such as P123 ((EO)20(PO)70(EO)20) or F127 ((EO)106(PO)70(EO)106), as the structure-directing agent. The MPS thin film prepared using P123 as the mesoporous template and treated via vacuum ultraviolet (VUV) irradiation to remove the triblock copolymer had a more uniform pore array than that of the corresponding film prepared via thermal treatment. Protein adsorption and enzyme-linked immunosorbent assay (ELISA) on the synthesized MPS thin films were also investigated. VUV-irradiated MPS thin films adsorbed a smaller quantity of protein A than the thermally treated films; however, the human immunoglobulin G (IgG) binding efficiency was higher on the former. In addition, protein A–IgG specific binding on MPS thin films was achieved without using a blocking reagent; i.e., nonspecific adsorption was inhibited by the uniform pore arrays of the films. Furthermore, VUV-irradiated MPS thin films exhibited high sensitivity for ELISA testing, and cytochrome c adsorbed on the MPS thin films exhibited high catalytic activity and recyclability. These results suggest that MPS thin films are attractive platforms for the development of novel biosensors. [Display omitted] •VUV-treated MPS thin films with removed polymer had uniform pore.•VUV-treated MPS thin films exhibited high sensitivity by ELISA.•Cytochrome c showed the catalytic activity and recyclability on synthesized films.