Adipo-myokine Irisin, a Promising Antioxidant against Nicotine Induced Oxidative Stress in BALB/c mice

• Published in: Pakistan journal of medical sciences Vol. 40; no. 1Part-I; pp. 115 - 119
• Main Authors: Imran, Madiha, Ahsin, Sadia, Saeed, Gule-Naghma, Ashraf, Hira
• Format: Journal Article
• Language: English
• Published: Pakistan Knowledge Bylanes 01.01.2024; AsiaNet Pakistan (Pvt) Ltd; Professional Medical Publications
• Subjects: Animals; Antioxidants; Biosynthesis; Diabetes; Enzymes; Exercise; Fenofibrate; Free radicals (Chemistry); Homeostasis; Insecticides; Ischemia; Lipid peroxidation; Lipids; Metabolism; Nicotine; Original; Oxidative stress; Pathogenesis; Tobacco; Type 2 diabetes; United Kingdom > UK; Islamabad Pakistan; Pakistan; Lipid peroxidation; Irisin; Oxidative stress; Antioxidant enzymes; Nicotine
• ISSN: 1682-024X; 1681-715X
• DOI: 10.12669/pjms.40.1.8140

To determine the role of r-irisin in attenuating nicotine-induced oxidative stress by estimating serum oxidative stress markers and antioxidant enzymes in BALB/c mice. This 18 month experimental study was carried out at Foundation University Islamabad and National Institute of Health starting in 2020. Thirty healthy BALB/c mice were divided into three groups. Group-I (control group) received normal saline 1ml/kg body weight intra-peritoneally daily for 28 days. Experimental group, Group-II received nicotine 2mg/kg body weight intra-peritoneally, for 28 days to induce oxidative stress. Experimental Group-III was given r-irisin 0.5 μg/g body weight/day via tail vein injection, for the last 14 days in addition to intraperitoneal nicotine for 28 days. On 29 day, intra-cardiac blood samples were taken for estimation of serum antioxidant enzymes [Superoxide dismutase (SOD), Reduced Glutathione (GSH) and Catalases (CAT)], and Thiobarbituric Acid-Reactive Substances (TBARS) levels as lipid peroxidation marker using ELISA. SPSS version 24 was used for statistical analysis. Significant difference in parameters across groups was calculated using one way ANOVA. -value of < 0.05 was considered significant. Group-II showed statistically significant increase in serum lipid peroxidation marker (TBARS) levels (p ) and reduction in serum anti-oxidative enzymes (SOD, CAT, GSH) levels (p ) as compared to Group-I. In Group-III, with co administration of r-irisin, significant improvement in antioxidant enzymes levels and reduction in TBARS levels was observed (p ) as compared to Group-II. Irisin ameliorates nicotine induced oxidative stress by improving serum anti-oxidant enzyme levels and reducing serum lipid peroxidation marker.