CRITICAL EVALUATION OF DIFFERENT METHODS FOR MEASURING THE FUNCTIONAL ACTIVITY OF ANTIBODIES AGAINST MALARIA BLOOD STAGE ANTIGENS

Antibodies are thought to be the primary immune effectors in the defense against erythrocytic stage Plasmodium falciparum. Thus, malaria vaccines directed to blood stages of infection are evaluated based on their ability to induce antibodies with anti-parasite activity. Such antibodies may have diff...

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Published inThe American journal of tropical medicine and hygiene Vol. 75; no. 3; pp. 437 - 442
Main Authors BERGMANN-LEITNER, ELKE S, DUNCAN, ELIZABETH H, MULLEN, GREGORY E, BURGE, JOHN ROBERT, KHAN, FARHAT, LONG, CAROLE A, ANGOV, EVELINA, LYON, JEFFREY A
Format Journal Article
LanguageEnglish
Published Lawrence, KS ASTMH 01.09.2006
Allen Press
Subjects
Animals
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antigens, Protozoan - blood
Antigens, Protozoan - immunology
Biological and medical sciences
Coloring Agents
Flow Cytometry
Human protozoal diseases
Humans
Immune Sera
Infectious diseases
L-Lactate Dehydrogenase - metabolism
Malaria
Malaria Vaccines - immunology
Medical sciences
Parasitic diseases
Plasmodium falciparum
Plasmodium falciparum - immunology
Protozoal diseases
Protozoa
Apicomplexa
Protozoal disease
Malaria
Antibody
Parasitosis
In vitro
Blood
Infection
Antigen
Plasmodium
Technology
Tropical medicine
Measurement method
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Summary:Antibodies are thought to be the primary immune effectors in the defense against erythrocytic stage Plasmodium falciparum. Thus, malaria vaccines directed to blood stages of infection are evaluated based on their ability to induce antibodies with anti-parasite activity. Such antibodies may have different effector functions (e.g., inhibition of invasion or inhibition of parasite growth/development) depending on the target antigen. We evaluated four methods with regards to their ability to differentiate between invasion and/or growth inhibitory activities of antibodies specific for two distinct blood stage antigens: AMA1 and MSP1(42). We conclude that antibodies induced by these vaccine candidates have different modes of action that vary not only by the antigen, but also by the strain of parasite being tested. Analysis based on parasitemia and viability was essential for defining the full range of anti-parasite activities in immune sera.
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2006.75.437