Ribosome Biogenesis Modulates Ty1 Copy Number Control in Saccharomyces cerevisiae

• Published in: Genetics (Austin) Vol. 207; no. 4; pp. 1441 - 1456
• Main Authors: Ahn, Hyo Won, Tucker, Jessica M, Arribere, Joshua A, Garfinkel, David J
• Format: Journal Article
• Language: English
• Published: United States Genetics Society of America 01.12.2017
• Subjects: Assembly; Biosynthesis; Cell cycle; Chromosome rearrangements; Cofactors; Copy number; Copy number control; Defects; DNA methylation; Gag protein; Gene expression; Genetics; Genomes; Genomics; Investigations; Localization; Mutants; Numerical controls; Packaging; Proteins; Ribonucleic acid; RNA; RNA polymerase; Saccharomyces cerevisiae; Saccharomyces paradoxus; Screens; Translation initiation; Transposition; Transposons; Viruses; Yeast; restriction factor; Saccharomyces; Ty1 retrotransposon; Loc1; ribosome biogenesis
• ISSN: 1943-2631; 0016-6731; 1943-2631
• DOI: 10.1534/genetics.117.300388

Transposons can impact the host genome by altering gene expression and participating in chromosome rearrangements. Therefore, organisms evolved different ways to minimize the level of transposition. In and its close relative , Ty1 copy number control (CNC) is mediated by the self-encoded restriction factor p22, which is derived from the capsid gene and inhibits virus-like particle (VLP) assembly and function. Based on secondary screens of Ty1 cofactors, we identified , a RNA localization/ribosome biogenesis gene that affects Ty1 mobility predominantly in strains harboring Ty1 elements. Ribosomal protein mutants Δ and Δ displayed similar CNC-specific phenotypes as Δ, suggesting that ribosome biogenesis is critical for CNC. The level of Ty1 mRNA and Ty1 internal (Ty1i) transcripts encoding p22 was altered in these mutants, and displayed a trend where the level of Ty1i RNA increased relative to full-length Ty1 mRNA. The level of p22 increased in these mutants, and the half-life of p22 also increased in a Δ mutant. Transcriptomic analyses revealed small changes in the level of Ty1 transcripts or efficiency of translation initiation in a Δ mutant. Importantly, a Δ mutant had defects in assembly of Gag complexes and packaging Ty1 RNA. Our results indicate that defective ribosome biogenesis enhances CNC by increasing the level of p22, and raise the possibility for versatile links between VLP assembly, its cytoplasmic environment, and a novel stress response.