Spatial heterogeneity in a deep artificial lake plankton community revealed by PCR-DGGE fingerprinting

• Published in: Chinese journal of oceanology and limnology Vol. 33; no. 3; pp. 624 - 635
• Main Author: 李强 赵越 张旭 魏雨泉 邱琳琳 魏自民 李富恒
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.05.2015; Science Press; Springer Nature B.V
• Subjects: Actinobacteria; Ammonia; Analysis; Analytical methods; Artificial lakes; Bacillariophyceae; Bacillariophyta; Biology; China; Ciliophora; Cluster analysis; Cosmopolitan species; Cryptophyceae; Cryptophycophyta; Cryptophyta; Cyanobacteria; denaturing gradient gel electrophoresis; Divergence; Earth and Environmental Science; Earth Sciences; Ecology; Electrophoresis; Environmental factors; Fingerprinting; Freshwater; Gels; GenBank; Genes; Heterogeneity; Inland water environment; Inland waters; Lakes; nitrates; nitrites; nitrogen content; Nucleotide sequence; Oceanography; Patchiness; PCR; PCR-DGGE; phylotype; Plankton; Proteobacteria; Redundancy; ribosomal RNA; Rotifera; rRNA 16S; rRNA 18S; rRNA基因; Sampling; Sequence analysis; Spatial heterogeneity; Stramenopiles; Studies; Water temperature; 人工湖; 变性梯度凝胶电泳; 指纹; 浮游生物群落; 空间异质性; China; China, People's Rep., Jilin Prov., Songhua L; China, People's Rep; Songhua Lake; redundancy analysis; plankton community composition; artificial lake; denaturing gradient gel electrophoresis (DGGE)
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-015-4184-9

To explore the spatial heterogeneity of plankton communities in a deep artificial lake （Songhua Lake, China）, samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis （DGGE） analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes （84%） exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla （Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera）. Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis （RDA）. The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.