Temperature-regulated metabolites of Serratia marcescens inhibited reproduction of pinewood nematode Bursaphelenchus xylophilus

• Published in: iScience Vol. 26; no. 7; p. 107082
• Main Authors: Zhang, Yanfen, Gu, Xiaoting, Huang, Chenying, Zhou, Jiao, Shi, Juan, Zhao, Lilin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 21.07.2023; Elsevier
• Subjects: Biological sciences; Evolutionary biology; Iochemistry; Evolutionary biology; Iochemistry; Biological sciences
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2023.107082

The pinewood nematode Bursaphelenchus xylophilus is an invasive and destructive pathogen in forestry. Serratia marcescens AHPC29 was previously found to have nematicidal activity on B. xylophilus. The effect of AHPC29 growth temperature on B. xylophilus inhibition is unknown. Here we show that AHPC29 cultured at 15°C or 25°C, but not 37°C, inhibited B. xylophilus reproduction. Metabolomic analysis found 31 up-regulated metabolites as potential effective substances in this temperature-related difference, with five of them were tested to be effective in inhibiting B. xylophilus reproduction. Among the five metabolites, salsolinol was further verified in bacterial cultures with effective inhibition concentrations. This study found the inhibition of S. marcescens AHPC29 on B. xylophilus reproduction was temperature regulated and the differently expressed metabolites salsolinol played roles in this temperature-regulated effect, which implies the capability of S. marcescens and its metabolites as promising new agents for the management of B. xylophilus. [Display omitted] •Effects of Serratia marcescens on pinewood nematode differed among culture temperatures•S. marcescens cultures at 15°C and 25°C inhibited Bursaphelenchus xylophilus reproduction•Culture temperature affected metabolite profiles of S. marcescens•Salsolinol, a metabolite of S. marcescens, contributed to its inhibition effect Iochemistry; Biological sciences; Evolutionary biology