Resistance training restores the gene expression of molecules related to fat oxidation and lipogenesis in the liver of ovariectomized rats

• Published in: European journal of applied physiology Vol. 112; no. 4; pp. 1437 - 1444
• Main Authors: Domingos, Mateus Moraes, Rodrigues, Maria Fernanda Cury, Stotzer, Uliana Sbeguen, Bertucci, Danilo Rodrigues, Souza, Markus Vinicius Campos, Marine, Diego Adorna, Gatto, Camila do Valle Gomes, de Araújo, Heloisa Sobreiro Selistre, de Andrade Perez, Sérgio Eduardo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2012; Springer; Springer Nature B.V
• Subjects: 3-Hydroxyacyl CoA Dehydrogenases - genetics; 3-Hydroxyacyl CoA Dehydrogenases - metabolism; Acetyl-CoA Carboxylase - genetics; Acetyl-CoA Carboxylase - metabolism; Analysis; Animal subjects; Animals; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Carnitine O-Palmitoyltransferase - genetics; Carnitine O-Palmitoyltransferase - metabolism; Dehydrogenases; Endurance; Enzymes; Estrogen; Estrogens; Estrogens - deficiency; Fatty acids; Fatty Liver - genetics; Fatty Liver - metabolism; Fatty Liver - prevention & control; Female; Fitness training programs; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation; Homeostasis; Human Physiology; Lipid Metabolism - genetics; Lipids; Lipogenesis - genetics; Liver; Liver - metabolism; Metabolism; Muscle Contraction; Muscle, Skeletal - metabolism; Occupational Medicine/Industrial Medicine; Oophorectomy; Original Article; Ovariectomy; Oxidation; Oxidation-Reduction; Physiology; PPAR alpha - genetics; PPAR alpha - metabolism; Preventive health; Rats; Rats, Sprague-Dawley; Real-Time Polymerase Chain Reaction; Resistance exercise; Resistance Training; Reverse Transcriptase Polymerase Chain Reaction; Sports Medicine; Stearoyl-CoA Desaturase - genetics; Stearoyl-CoA Desaturase - metabolism; Sterol Regulatory Element Binding Protein 1 - genetics; Sterol Regulatory Element Binding Protein 1 - metabolism; Sterols; Strength training; Time Factors; Transcription factors; Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports; Fat oxidation; Hepatic steatosis; Ovariectomy; Lipogenesis; Resistance training; Human; Physical training; Rat; Digestive system; Liver; Rodentia; Hepatic disease; Gene expression; Vertebrata; Mammalia; Fatty liver; Fat; Digestive diseases; Oxidation
• ISSN: 1439-6319; 1439-6327
• DOI: 10.1007/s00421-011-2098-6

Decreased levels of estrogen are associated with hepatic steatosis (HS), through changes in gene expression of molecules related to fat oxidation and lipogenesis. Both resistance training (RT) and endurance training (ET) prevent HS in ovariectomized (Ovx) rats. However, the molecular events associated with this process were only investigated for ET, but not for RT. Thus, the aim of this study was to investigate the effects of Ovx and RT on the gene expression of molecules related to fat oxidation and lipogenesis in the liver of rats. Sprague–Dawley adult female rats were grouped into four ( n  = 6 per group): sham-operated sedentary (Sham-Sed); Ovx sedentary (Ovx-Sed); sham-Rt and Ovx-Rt. A 10-week RT period, during which the animals climbed a 1.1-m vertical ladder with weights attached to their tails, was used. The sessions were performed three times a week, with 4–9 climbs and 8–12 dynamic movements per climb. Gene expression was analyzed by RT-PCR by the ∆∆Ct method. The estrogen deficiency associated with ovariectomy decreased the gene expression of molecules related to fat oxidation, carnitine palmitoyltransferase I (53%) and β-hydroxyacyl-CoA dehydrogenase (27%), and increased molecules related to lipogenesis, sterol regulatory element-binding protein-1c (106%), acetyl-CoA carboxylase (ACC) (72%) and stearoyl CoA desaturase-1 (109%). With the exception of ACC, the ovariectomy-induced changes in the expression of these molecules were restored by RT. The present results indicate that RT has important effects on the prevention of HS in Ovx animals, through changes in gene expression of molecules related to hepatic lipid metabolism.