Pharmacological and signalling properties of a D2-like dopamine receptor (Dop3) in Tribolium castaneum

• Published in: Insect biochemistry and molecular biology Vol. 56; pp. 9 - 20
• Main Authors: Verlinden, Heleen, Vleugels, Rut, Verdonck, Rik, Urlacher, Elodie, Vanden Broeck, Jozef, Mercer, Alison
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2015
• Subjects: Amine; Animals; CHO Cells; Cricetulus; Cyclic AMP - metabolism; Dopamine; Dopamine Agonists - pharmacology; Dopamine Antagonists - pharmacology; Evolution; G protein-coupled receptor (GPCR); HEK293 Cells; Humans; Insect; Molecular Sequence Data; Pharmacology; Real-Time Polymerase Chain Reaction; Receptors, Dopamine - analysis; Receptors, Dopamine - metabolism; Receptors, G-Protein-Coupled - metabolism; Sequence Analysis, Protein; Signal Transduction; Signalling; Tribolium - drug effects; Tribolium - genetics; Tribolium - metabolism; Tribolium castaneum; Amine; Dopamine; G protein-coupled receptor (GPCR); Evolution; Pharmacology; Signalling; Insect
• ISSN: 0965-1748; 1879-0240
• DOI: 10.1016/j.ibmb.2014.11.002

Dopamine is an important neurotransmitter in the central nervous system of vertebrates and invertebrates. Despite their evolutionary distance, striking parallels exist between deuterostomian and protostomian dopaminergic systems. In both, signalling is achieved via a complement of functionally distinct dopamine receptors. In this study, we investigated the sequence, pharmacology and tissue distribution of a D2-like dopamine receptor from the red flour beetle Tribolium castaneum (TricaDop3) and compared it with related G protein-coupled receptors in other invertebrate species. The TricaDop3 receptor-encoding cDNA shows considerable sequence similarity with members of the Dop3 receptor class. Real time qRT-PCR showed high expression in both the central brain and the optic lobes, consistent with the role of dopamine as neurotransmitter. Activation of TricaDop3 expressed in mammalian cells increased intracellular Ca2+ signalling and decreased NKH-477 (a forskolin analogue)-stimulated cyclic AMP levels in a dose-dependent manner. We studied the pharmacological profile of the TricaDop3 receptor and demonstrated that the synthetic vertebrate dopamine receptor agonists, 2 – amino- 6,7 – dihydroxy – 1,2,3,4 – tetrahydronaphthalene hydrobromide (6,7-ADTN) and bromocriptine acted as agonists. Methysergide was the most potent of the antagonists tested and showed competitive inhibition in the presence of dopamine. This study offers important information on the Dop3 receptor from Tribolium castaneum that will facilitate functional analyses of dopamine receptors in insects and other invertebrates. [Display omitted] •The sequence encoding a D2-like dopamine receptor of T. castaneum was amplified and examined.•TricaDop3 was dose-dependently activated by dopamine in mammalian cell lines.•Bromocriptine and 6,7-ADTN acted as full agonists on TricaDop3.•Methysergide showed to be a potent competitive inhibitor of TricaDop3.•Activation of TricaDop3 decreased intracellular cAMP levels and induced elevated calcium levels in mammalian cells.