Isocoumarin formation by heterologous gene expression and modification by host enzymes

• Published in: Organic & biomolecular chemistry Vol. 18; no. 26; pp. 4946 - 4948
• Main Authors: Xiang, Pan, Ludwig-Radtke, Lena, Yin, Wen-Bing, Li, Shu-Ming
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 08.07.2020
• Subjects: Enzymes; Fungi; Gene expression; Metabolites; NMR; Nuclear magnetic resonance; Polyketide synthase

Heterologous expression has been proven to be a successful strategy for the identification of metabolites encoded by cryptic/silent genes. Expression of a nonreducing polyketide synthase (NR-PKS) gene from Penicillium crustosum in Aspergillus nidulans led to the accumulation of three isocoumarins 1-...