Isocoumarin formation by heterologous gene expression and modification by host enzymes

Heterologous expression has been proven to be a successful strategy for the identification of metabolites encoded by cryptic/silent genes. Expression of a nonreducing polyketide synthase (NR-PKS) gene from Penicillium crustosum in Aspergillus nidulans led to the accumulation of three isocoumarins 1-...

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Published inOrganic & biomolecular chemistry Vol. 18; no. 26; pp. 4946 - 4948
Main Authors Xiang, Pan, Ludwig-Radtke, Lena, Yin, Wen-Bing, Li, Shu-Ming
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 08.07.2020
Subjects
Enzymes
Fungi
Gene expression
Metabolites
NMR
Nuclear magnetic resonance
Polyketide synthase
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Summary:Heterologous expression has been proven to be a successful strategy for the identification of metabolites encoded by cryptic/silent genes. Expression of a nonreducing polyketide synthase (NR-PKS) gene from Penicillium crustosum in Aspergillus nidulans led to the accumulation of three isocoumarins 1-3 . Feeding experiments revealed that the PKS product 1 can be converted by the host enzymes to its hydroxylated ( 2 ) and methylated ( 3 ) derivatives. These results provided one additional example that unexpected further modifications of an enzyme product can take place in a heterologous host. The polyketide synthase product was converted to its methylated and hydroxylated derivatives by host endogenous enzymes.
Bibliography:10.1039/d0ob00989j
Electronic supplementary information (ESI) available: Experimental procedures, NMR data and spectra. See DOI
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ISSN:1477-0520
1477-0539
1477-0539
DOI:10.1039/d0ob00989j