Allelotype analysis of adenocarcinoma of the gastric cardia

• Published in: British journal of cancer Vol. 76; no. 11; pp. 1455 - 1465
• Main Authors: GLEESON, C. M, SLOAN, J. M, MCGUIGAN, J. A, RITCHIE, A. J, WEBER, J. L, RUSSELL, S. E. H
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing Group 1997
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - pathology; Alleles; Biological and medical sciences; Cardia; Chromosome Aberrations; Chromosome Breakage; Chromosome Mapping; Chromosomes, Human, Pair 4; Chromosomes, Human, Pair 6; DNA, Neoplasm - genetics; Gastroenterology. Liver. Pancreas. Abdomen; Genetic Markers; Humans; Medical sciences; Microsatellite Repeats; Polymorphism, Genetic; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Adenocarcinoma; Human; Stomach; Breakpoint; Typing; Cardia; Chromosome B4; Malignant tumor; Chromosome C6; Allele; Gene; Cytogenetics; Digestive diseases; Genetics; Localization; Gastric disease
• ISSN: 0007-0920; 1532-1827
• DOI: 10.1038/bjc.1997.578

To identify chromosomal loci involved in the development of proximal gastric adenocarcinoma, this study delineated the pattern of allelic imbalance in a series of 38 adenocarcinomas arising in the gastric cardia. A total of 137 microsatellite markers covering all autosomal arms, excluding acrocentric arms, were analysed. A mean of 35 out of a total of 39 chromosomal arms studied were informative for each patient. The tumour group demonstrated a high level of allelic imbalance, with an observed median fractional allelic imbalance of 0.47 for the 29 intestinal-type adenocarcinomas and 0.54 for the nine diffuse-type adenocarcinomas. Allelic imbalance was detected in >50% of informative cases in both histological subtypes on a number of chromosomal arms. In the intestinal subtype, these included, 3p (61%), 4q (71%), 5q (59%), 8p (60%), 9p (65%), 9q (83%), 12q (52%), 13q (52%), 17p (78%) and 18q (70%). A higher incidence of allelic imbalance was detected on chromosome 16q in tumours of the diffuse type relative to those of the intestinal type. A more detailed mapping on chromosomes 4q and 6q identified a number of cases with subchromosomal breakpoints. In conclusion, this analysis has indicated regions of the genome potentially involved in the development of proximal gastric carcinomas.