Lipoxygenase inhibitory activity of Cuspidaria pulchra and isolated compounds

• Published in: Natural product research Vol. 29; no. 11; pp. 1083 - 1086
• Main Authors: Alvarenga, Tavane A., Bertanha, Camila S., de Oliveira, Pollyanna F., Tavares, Denise C., Gimenez, Valéria M.M., Silva, Márcio L.A., Cunha, Wilson R., Januário, Ana H., Pauletti, Patrícia M.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.06.2015
• Subjects: 15-lipoxygenase inhibitory activity; Arrabidaea; Bignoniaceae - chemistry; Caffeic Acids - chemistry; Caffeic Acids - isolation & purification; Caffeic Acids - pharmacology; cytotoxic activity; Glucosides - chemistry; Glucosides - isolation & purification; Lipoxygenase Inhibitors - pharmacology; Luteolin - chemistry; Luteolin - isolation & purification; Molecular Structure; Phenols - chemistry; Phenols - isolation & purification; Plant Components, Aerial - chemistry; Plant Extracts - pharmacology; 15-lipoxygenase inhibitory activity; Arrabidaea; cytotoxic activity
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2014.981182

This work evaluated the in vitro inhibitory activity of the crude ethanolic extract from the aerial parts of Cuspidaria pulchra (Cham.) L.G. Lohmann against 15-lipoxygenase (15-LOX). The bioassay-guided fractionation of the n-butanol fraction, which displayed the highest activity, led to the isolation of three compounds: caffeoylcalleryanin (1), verbascoside (2) and 6-hydroxyluteolin-7-O-β-glucoside (3). Assessment of the ability of the isolated compounds to inhibit 15-LOX revealed that compounds 1, 2 and 3 exerted strong 15-LOX inhibitory activity; IC 50 values were 1.59, 1.76 and 2.35 μM respectively. The XTT assay showed that none of the isolated compounds seemed to be significantly toxic.