Supercritical carbon dioxide extraction of turmeric oil from Curcuma longa Linn and purification of turmerones

• Published in: Separation and purification technology Vol. 47; no. 3; pp. 119 - 125
• Main Authors: Chang, Li-Hsun, Jong, Ting-Ting, Huang, Ho-Shin, Nien, Yung-Feng, Chang, Chieh-Ming J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 2006; Elsevier Science
• Subjects: Applied sciences; Chemical engineering; Exact sciences and technology; Extraction; Liquid-liquid extraction; Purification; Response-surface methodology; Supercritical carbon dioxide; Turmeric oil; Turmerones; Turmeric oil; Extraction; Supercritical carbon dioxide; Purification; Response-surface methodology; Turmerones; Carbon dioxide; HPLC chromatography; Purity; Extract; Supercritical state; Optimization; Powder; Qualification; Production; Silica gel; Liquid solid chromatography; Supercritical fluid extraction
• ISSN: 1383-5866; 1873-3794
• DOI: 10.1016/j.seppur.2005.06.018

This study investigates separation and purification of three turmerones from the turmeric oil extracted by supercritical carbon dioxide. Nearly, 800 g of supercritical carbon dioxide extraction of 75 g of 0.42 mm turmeric powders at 333 K and 300 bar obtained 6.98 wt% turmeric oil within 2.5 h. A response-surface methodology is adopted for optimizing supercritical carbon dioxide extraction to disclose that the combination of 320 K and 26 MPa gives an optimum production of turmeric oil containing 71 wt% purity of three turmerones. Subsequently, a normal phase silica gel 60 column is employed to separate and purify three major turmerones from the turmeric oil extracted by supercritical carbon dioxide. The 86 wt% purity of Ar-turmerone and 81 wt% purity of α + β-turmerone have been separated, collected, and identified by liquid–solid chromatography, NMR qualification, and HPLC quantification, respectively.