Does insulin release kinins in rats?

• Published in: European journal of pharmacology Vol. 525; no. 1; pp. 154 - 160
• Main Authors: Damas, Jacques, Garbacki, Nancy, Lefèbvre, Pierre J.
• Format: Journal Article Web Resource
• Language: English
• Published: Amsterdam Elsevier B.V 21.11.2005; Elsevier; Elsevier Science Bv
• Subjects: Angiotensin-converting enzyme inhibitor; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Biological and medical sciences; Blood Glucose - analysis; Bradykinin; Bradykinin - analogs & derivatives; Bradykinin - pharmacology; Bradykinin Receptor Antagonists; Bradykinin/analogs & derivatives/pharmacology; Captopril - pharmacology; Female; Glucose; Glucose - pharmacology; Human health sciences; Hyperglycemia - chemically induced; Hyperglycemia - metabolism; Hyperglycemia/chemically induced/metabolism; Insulin; Insulin - pharmacology; Isometric Contraction; Kinin; Kinins - metabolism; Medical sciences; Pharmacie, pharmacologie & toxicologie; Pharmacology. Drug treatments; Pharmacy, pharmacology & toxicology; Rat uterus; Rats; Rats, Wistar; Sciences de la santé humaine; Uterine Contraction - drug effects; Rat uterus; Angiotensin-converting enzyme inhibitor; Glucose; Insulin; Bradykinin; Kinin; Pancreatic hormone; Vasodilator agent; Rat; Peptide hormone; Rodentia; Neuropeptide; Vertebrata; Mammalia; Uterus; Animal; Female genital system; ACE inhibitor; Release
• ISSN: 0014-2999; 1879-0712
• DOI: 10.1016/j.ejphar.2005.10.001

Rat uterus maintained in situ was used as a bioassay of kinins possibly released in vivo by hyperglycaemia or insulin. Intravenous injections of bradykinin induced contractions of rat uterus which were suppressed by HOE 140, a bradykinin B 2 receptor antagonist. Des-Arg 9-bradykinin, a kinin B 1 receptor agonist, did not elicit any response. After propranolol, the effects of bradykinin were enhanced and dose-dependent. This potentiation did not appear in adrenalectomized rats. Captopril, an angiotensin-converting enzyme (ACE) inhibitor, largely increased the effects of bradykinin. In animals pretreated with propranolol, captopril and atosiban, an oxytocin antagonist, intravenous infusion of glucose induced hyperglycaemia and after a delay increased the uterine contractile activity. This contractile effect of glucose was abolished by HOE 140. Infusion of insulin with glucose induced contractions of the uterus. These responses did not appear or were suppressed by HOE 140 or by soya bean trypsin inhibitor (SBTI), a plasma kallikrein inhibitor. These results are direct evidence that insulin induces a release of kinins.