Isolation, structural elucidation and anti-inflammatory activity of astragalin, ( − )hinokinin, aristolactam I and aristolochic acids (I & II) from Aristolochia indica

• Published in: Natural product research Vol. 28; no. 17; pp. 1413 - 1417
• Main Authors: Desai, Dattatraya C., Jacob, Jeenu, Almeida, Asha, Kshirsagar, Rajendra, Manju, S.L.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2014
• Subjects: ( − )hinokinin; 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - chemistry; 4-Butyrolactone - isolation & purification; 4-Butyrolactone - pharmacology; Anti-Inflammatory Agents; aristolactam I; Aristolochia - chemistry; Aristolochia indica; Aristolochic Acids - chemistry; Aristolochic Acids - isolation & purification; Aristolochic Acids - pharmacology; astragalin; Benzodioxoles; Chromatography, High Pressure Liquid; Dioxoles - chemistry; Dioxoles - isolation & purification; Dioxoles - pharmacology; IL-6; Interleukin-6 - antagonists & inhibitors; Kaempferols - chemistry; Kaempferols - isolation & purification; Kaempferols - pharmacology; Lignans - chemistry; Lignans - isolation & purification; Lignans - pharmacology; NFκB; TNFα; TNFα; NFκB; ( − )hinokinin; astragalin; aristolactam I; Aristolochia indica; IL-6
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2014.905563

Astragalin was isolated for the first time along with ( − )hinokinin, aristolactam I and aristolochic acids (I & II) from the extracts of Aristolochia indica L. using a new, efficient preparative HPLC method. A reversed-phase HPLC method of analysis was developed to analyse the isolated compounds. The crude extracts and the isolated compounds were tested for their anti-inflammatory potential. We report here for the first time the anti-inflammatory effects of ( − )hinokinin and aristolactam I against IL-6 (IC 50  = 20.5 ± 0.5 and 52 ± 8 μM) and TNFα (IC 50  = 77.5 ± 27.5 and 116.8 ± 83.25 μM), respectively. ( − )Hinokinin exerted its anti-inflammatory effects via NFκB-dependent mechanism whereas aristolactam I may be effective via a mechanism independent of NFκB.