Factors that contribute to the transneuronal spread of herpes simplex virus
In viral encephalitis and retinal necrosis, different herpes simplex virus (HSV) strains spread between neurons in the central nervous system (CNS) by distinctly different routes. The steps of viral infection and spread in a single neuron type and nearby glial cells in vivo have been determined for...
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Published in | Journal of neuroscience research Vol. 49; no. 4; pp. 485 - 496 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York
John Wiley & Sons, Inc
15.08.1997
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Subjects | |
Online Access | Get full text |
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Summary: | In viral encephalitis and retinal necrosis, different herpes simplex virus (HSV) strains spread between neurons in the central nervous system (CNS) by distinctly different routes. The steps of viral infection and spread in a single neuron type and nearby glial cells in vivo have been determined for three different strains of HSV (F, H129, and McIntyre‐B). The corneas of mice were inoculated with equivalent titers of the strains. Two to 5 days later, the animals were killed. The spread of viral proteins within trigeminal cells was examined using immuno‐ and electron microscopy and Western blots with anti‐HSV polyclonal antiserum. McIntyre‐B virus infection resulted in fewer labeled ganglion cells, possibly as a result of reduced viral production in the corneal epithelium or trigeminal ganglion cells. Although the McIntyre‐B strain was at least as, if not more efficient, at retrograde transport than the other strains, the amount of McIntyre‐B virus that was transported in the trigeminal roots in an anterograde direction was significantly less than the other strains. Uptake by ganglionic satellite cells was qualitatively similar for the three strains, but maturation and release of virus from satellite cells to other neurons were reduced in the McIntyre‐B strain. These characteristics may account for the preferential retrograde transneuronal spread of McIntyre‐B strain. J. Neurosci. Res. 49:485–496, 1997. © 1997 Wiley‐Liss, Inc. |
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Bibliography: | ArticleID:JNR9 NIH - No. EY08773; No. EY06322 Research to Prevent Blindness ark:/67375/WNG-VBXHR7F5-B That Man May See, Inc istex:1E8BA1A03ADFBC060F0BAB49204262893E4A1F4B ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0360-4012 1097-4547 |
DOI: | 10.1002/(SICI)1097-4547(19970815)49:4<485::AID-JNR9>3.0.CO;2-4 |