An efficient extraction method from blood clots for studies requiring both host and viral DNA

The clot from blood is usually discarded after the collection of serum. Yet, it contains nucleated white blood cells and substantial serum. Here, we have compared four methods to enable quick and efficient extraction of human genomic and viral DNA from clotted blood. Two of these methods, a phenol‐b...

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Bibliographic Details
Published inJournal of viral hepatitis Vol. 7; no. 3; pp. 241 - 243
Main Authors Basuni, A A, Butterworth, L A, Cooksley, G, Locarnini, S, Carman, W F
Format Journal Article
LanguageEnglish
Published Oxford UK Blackwell Science Ltd 01.05.2000
Subjects
blood clots
Blood Coagulation
Blood Specimen Collection
DNA - isolation & purification
DNA extraction
DNA, Viral - isolation & purification
hepatitis B virus
human β-globin
Humans
Indicators and Reagents
Phenol
Reagent Kits, Diagnostic
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Summary:The clot from blood is usually discarded after the collection of serum. Yet, it contains nucleated white blood cells and substantial serum. Here, we have compared four methods to enable quick and efficient extraction of human genomic and viral DNA from clotted blood. Two of these methods, a phenol‐based in‐house method and Tripure isolation reagent, only achieved a low polymerase chain reaction (PCR) yield. In contrast, the QIAamp blood kit and the High Pure Viral Nucleic Acid kit were equally efficient, with similar sensitivity to serum for extraction of viral DNA.
Bibliography:ark:/67375/WNG-6LC6C2RT-9
ArticleID:JVH206
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SourceType-Scholarly Journals-1
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ISSN:1352-0504
1365-2893
DOI:10.1046/j.1365-2893.2000.00206.x