Comparison of Two Convection-Aided Protein Adsorption Methods Using Porous Membranes and Perfusion Beads

• Published in: Biotechnology progress Vol. 12; no. 6; pp. 869 - 872
• Main Authors: Kubota, Noboru, Konno, Yoshitaka, Miura, Suguru, Saito, Kyoichi, Sugita, Kazuyuki, Watanabe, Kohei, Sugo, Takanobu
• Format: Journal Article
• Language: English
• Published: USA American Chemical Society 1996; American Institute of Chemical Engineers
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Others; Various methods and equipments; Ball; Elution; Adsorption; Porous membrane; Perfusion; Hollow fiber; Anion exchanger; Serum albumin; Chromatography; Comparative study
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp960076s

We describe results which serve as a guide in the selection of protein recovery techniques using two new chromatographic methods based on anion‐exchange interaction, i.e., membrane and perfusion chromatographies, which involve enhancement of protein transport by convective flow. Bovine serum albumin solution was permeated through a functionalized porous hollow‐fiber membrane and a bed charged with functionalized porous (perfusion) beads of identical volume. The pores of the membrane are surrounded by grafted polymer chains immobilizing anion‐exchange groups, whereas the throughpores of the beads are surrounded by the diffusive pores at the periphery of which anion‐exchange groups are immobilized. An 8‐fold increase in throughput of the protein using the porous membrane, 37 mg of BSA/mL/min, was achieved at a low operating pressure of 0.1 MPa, compared to that using the perfusion bed.