Poly(ε-caprolactone)/nano fluoridated hydroxyapatite scaffolds for bone tissue engineering: in vitro degradation and biocompatibility study

• Published in: Journal of materials science. Materials in medicine Vol. 23; no. 3; pp. 763 - 770
• Main Authors: Johari, N., Fathi, M. H., Golozar, M. A., Erfani, E., Samadikuchaksaraei, A.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2012; Springer; Springer Nature B.V
• Subjects: Biocompatibility; Biocompatible Materials; Biodegradable materials; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biotechnology; Bone and Bones - chemistry; Cell Line; Ceramics; Chemistry and Materials Science; Composites; Durapatite - chemistry; Fundamental and applied biological sciences. Psychology; Glass; Health. Pharmaceutical industry; Humans; Hydroxyapatite; Industrial applications and implications. Economical aspects; Materials Science; Medical sciences; Microscopy, Electron, Scanning; Miscellaneous; Natural Materials; Polyesters - chemistry; Polymer Sciences; Regenerative Medicine/Tissue Engineering; Spectroscopy, Fourier Transform Infrared; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Tissue Engineering; X-Ray Diffraction; Bone Tissue Engineering; Fluorine Content; Amorphous Calcium Phosphate; Tissue Engineering Scaffold; Tricalcium Phosphate; Biodegradation; Biological properties; Biodegradability; Tissue engineering; Hydroxyapatite; Scaffold; Lactone polymer; Osteoarticular system; Aliphatic polymer; Biocompatibility; Bone; Polycaprolactone; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-011-4528-8

In this study, biodegradation and biocompatibility of novel poly(ε-caparolactone)/nano fluoridated hydroxyapatite (PCL–FHA) scaffolds were investigated. The FHA nanopowders were prepared via mechanical alloying method and had a chemical composition of Ca 10 (PO 4 ) 6 OH 2– x F x (where x values were selected equal to 0.5 and 2.0). In order to fabricate PCL–FHA scaffolds, 10, 20, 30 and 40 wt% of the FHA were added to the PCL. The PCL–FHA scaffolds were produced by the solvent casting/particulate leaching using sodium chloride particles (with diameters of 300–500 μm) as the porogen. The phase structure, microstructure and morphology of the scaffolds were evaluated using X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy techniques. Porosity of the scaffolds was measured using the Archimedes’ Principle. In vitro degradation of PCL–FHA scaffolds was studied by incubating the samples in phosphate buffered saline at 37°C and pH 7.4 for 30 days. Moreover, biocompatibility was evaluated by MTT assay after seeding and culture of osteoblast-like cells on the scaffolds. Results showed that the osteoblast-like cells attached to and proliferated on PCL–FHA and increasing the porosity of the scaffolds increased the cell viability. Also, degradation rate of scaffolds were increased with increasing the fluorine content in scaffolds composition.