Urine 8-Hydroxyguanine (8-OHG) in Patients Undergoing Surgery for Colorectal Cancer

• Published in: Journal of investigative surgery Vol. 35; no. 3; pp. 591 - 597
• Main Authors: Pappas-Gogos, George, Tellis, Constantinos C., Tepelenis, Kostas, Vlachos, Konstantinos, Chrysos, Emmanuel, Tselepis, Alexandros D., Glantzounis, Georgios K.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.03.2022; Taylor & Francis Group
• Subjects: 8-hydroxyguanine; abdominal surgery; biomarkers; Colorectal cancer; Colorectal Neoplasms - surgery; DNA - metabolism; Guanine - analogs & derivatives; Humans; Oxidative Stress; RNA oxidation; Uric Acid; abdominal surgery; 8-hydroxyguanine; biomarkers; Colorectal cancer; RNA oxidation; uric acid; oxidative stress
• ISSN: 0894-1939; 1521-0553
• DOI: 10.1080/08941939.2021.1904466

Cellular RNA is less compact than DNA, more easily accessible to ROS and therefore could be more susceptible to oxidative damage. This study was conceived in order to analyze the RNA oxidative damage in the urine of patients undergoing operation for colorectal cancer (CRC), to compare with healthy controls, and correlate with the stage. The study population was constituted by a group of 147 patients and a group of 128 healthy controls. Urine and blood samples were collected before the colonoscopy in all participants and 24 hours post-operatively for those who underwent surgery. Urine 8-hydroxyguanine (8-OHG) was determined as marker of RNA oxidation, and serum uric acid (UA) as antioxidant marker. Preoperatively, 8-OHG (ng/ml) values of CRC patients were found to be significantly higher than those of controls (p = 0.001). More specifically, stages II/III had significantly higher 8-OHG values (p < 0.001 and p = 0.007) than stages 0/I. Post-operatively, 8-OHG values were similar to controls (p = 0.053). Preoperatively, UA values (mg/dl) were significantly lower (p = 0.001), while postoperatively were similar to controls (p = 0.069). Oxidative RNA damage occurs in CRC patients. Stages II/III are associated with higher values of 8-OHG than stages 0/I. 8-OHG could act as a marker for the identification of patients with advanced disease.