The Interaction Between MiR-141 and lncRNA-H19 in Regulating Cell Proliferation and Migration in Gastric Cancer

• Published in: Cellular physiology and biochemistry Vol. 36; no. 4; pp. 1440 - 1452
• Main Authors: Zhou, Xiaoying, Ye, Feng, Yin, Chengqiang, Zhuang, Ya, Yue, Ge, Zhang, Guoxin
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Cell Physiol Biochem Press GmbH & Co KG 01.01.2015
• Subjects: Base Sequence; Cell Line, Tumor; Cell Movement; Cell Proliferation; Gastric cancer; Gastric Mucosa - metabolism; Gene Expression Regulation, Neoplastic; H19; Homeodomain Proteins - genetics; Humans; MicroRNAs - genetics; MicroRNAs - metabolism; Migration; MiR-141; Neoplasm Invasiveness - genetics; Neoplasm Invasiveness - pathology; Original Paper; Proliferation; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Stomach - pathology; Stomach Neoplasms - genetics; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Transcription Factors - genetics; Zinc Finger E-box-Binding Homeobox 1; H19; MiR-141; Proliferation; Gastric cancer; Migration
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000430309

Background/Aims: Non-coding RNAs including miRNA and lncRNA had been reported to regulate gene expression and were both related to cancer progression. MicroRNA-141 (miR-141) has been reported to play a role in the epithelial to mesenchymal transition (EMT) process and H19 has also been demonstrated to promote malignancy in various cancers. We aimed to determine the correlation between miR-141 and H19 and their roles in gastric cancer in this study. Methods: H19 and miR-141 expression were detected by qRT-PCR. By bioinformatic analysis and luciferase assay we examined the correlation between H19 and miR-141 in vitro. Results: H19 expression was found to be inversely correlated to miR-141 expression in gastric cancer cells and tissues. H19 promotes malignancy including proliferation and invasion whereas miR-141 suppresses malignancy in human cancer cells. MiR-141 binds to H19 in a sequence specific manner, and suppresses H19 expression and functions including proliferation and invasion. MiR-141 could also regulate H19 target genes and miR-141 inhibitor restores H19 siRNA function, while H19 regulates miR-141 target gene ZEB1. Conclusion: These results were the first to demonstrate that H19 and miR-141 could compete with each other and affect their target genes in gastric cancer, which provide important clues for understanding the key roles of lncRNA-miRNA functional network in cancer.