Inducible protective processes in animal systems VI. Cross-adaptation and the influence of caffeine on the adaptive response in bone marrow cells of mouse

• Published in: Mutagenesis Vol. 15; no. 3; pp. 271 - 276
• Main Authors: Harish, S.K., Guruprasad, K.P., Mahmood, Riaz, Vasudev, V.
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.05.2000
• Subjects: Acclimatization; Animals; Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Caffeine - administration & dosage; Caffeine - pharmacology; Chemical mutagenesis; Chromosome Aberrations; DNA Damage; Ethyl Methanesulfonate - administration & dosage; Ethyl Methanesulfonate - pharmacology; Male; Medical sciences; Mice; Mutagenicity Tests; Mutagens - administration & dosage; Mutagens - pharmacology; Time Factors; Toxicology; Chromosomal aberration; CNS stimulant; Rodentia; Adaptive response; Respiratory analeptic; In vivo; Vertebrata; Mammalia; Cell line; Mouse; Bone marrow; High dose; Adaptation; Caffeine
• ISSN: 0267-8357; 1464-3804; 1464-3804
• DOI: 10.1093/mutage/15.3.271

The effect of caffeine (CAF) (a replicative DNA synthesis inhibitor) given as pre-, inter- and post-treatments on the ethyl methanesulfonate (EMS)-induced adaptive response in in vivo mouse bone marrow cells was studied in order to understand the influence of CAF on the adaptive response. The pre-treatment was given 4 h before a combined treatment with EMS (conditioning + challenge) and in another set CAF was given as a conditioning dose and 4 h later the cells were challenged with a high dose of EMS. In the inter-treatment, CAF (40 mg/kg body wt) was administered 2 or 4 h after the conditioning dose of EMS and 6 or 4 h later the cells were challenged with a high dose of EMS. Similarly, in the post-treatment experiments, CAF was injected 6, 12 or 18 h after a combined treatment with EMS. The results revealed that the pre-, inter- and post-treatments with CAF significantly reduced the frequency of chromosomal aberrations compared with the challenge and combined treatments with EMS. It is interesting to note that CAF pre-treatment resulted in a much greater reduction in chromosomal aberrations compared with the inter- and post-treatments. Thus, this is an example of cross-adaptation induced by CAF in EMS-treated in vivo mouse bone marrow cells and the results also demonstrate an influence of CAF on the adaptive response.