Organization and nucleotide sequence of carp gonadotropin α subunit genes

• Published in: Biochimica et biophysica acta Vol. 1129; no. 2; pp. 239 - 242
• Main Authors: Huang, Chang-Jen, Huang, Fore-Lien, Wang, Yi-Chi, Chang, Yea-Sha, Lo, Tung-Bin
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 06.01.1992; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Carps - genetics; Cloning, Molecular; Cyprinus carpio; DNA, Single-Stranded; Exons; Fundamental and applied biological sciences. Psychology; Genes. Genome; Glycoprotein hormone; Glycoprotein Hormones, alpha Subunit - genetics; Gonadotropins, Pituitary - genetics; Intron-exon boundary; Introns; Molecular and cellular biology; Molecular evolution; Molecular genetics; Molecular Sequence Data; Polymerase Chain Reaction; Recombinant DNA; Molecular evolution; GTHα; kb; Cyprinus carpio; GTH; Recombinant DNA; Intron-exon boundary; Glycoprotein hormone; cGTHα; bp; PCR; Polymerase chain reaction; Vertebrata; Mammalia; Gene; Nucleotide sequence; Pisces; Sequence alignment; Alpha-Peptide chain; Gene organization; Gonadotropin
• ISSN: 0167-4781; 0006-3002; 1879-2634
• DOI: 10.1016/0167-4781(92)90496-M

We have used PCR to amplify and align the sequence of two genes encoding cGTHα. Both genes comprise four exons and three introns. The organization of cGTHα genes is very similar to that of mammalian GTHα genes. However, the cGTHα genes only span a region of 1.2 kb which is much smaller than those mammalian GTHα genes.