Large scale biomimetic membrane arrays

• Published in: Analytical and bioanalytical chemistry Vol. 395; no. 3; pp. 719 - 727
• Main Authors: Hansen, Jesper S, Perry, Mark, Vogel, Jörg, Groth, Jesper S, Vissing, Thomas, Larsen, Marianne S, Geschke, Oliver, Emneús, Jenny, Bohr, Henrik, Nielsen, Claus H
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.10.2009; Springer-Verlag
• Subjects: Analytical Chemistry; Apertures; Array; Arrays; Bacillus - chemistry; Bacterial Outer Membrane Proteins - chemistry; Bacterial Toxins - chemistry; Biochemistry; Biomimetics - instrumentation; Black lipid membrane; Carbon dioxide; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Electrochemical Techniques; Equipment Design; Food Science; Gramicidin - chemistry; Hemolysin Proteins - chemistry; Laboratory Medicine; Lasers; Lipid Bilayers - chemistry; Lipids; Mathematical analysis; Membrane protein; Membrane Proteins - chemistry; Membrane-spanning peptides; Membranes; Membranes, Artificial; Monitoring/Environmental Analysis; Optical-electrical measurements; Original Paper; Partitions; Peptides, Cyclic - chemistry; Staphylococcus aureus - chemistry; Valinomycin - chemistry; Array; Optical–electrical measurements; Membrane protein; Membrane-spanning peptides; Black lipid membrane
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-009-3010-7

To establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical-electrical measurements. Functional lipid bilayers could easily and efficiently be established across CO₂ laser micro-structured 8 x 8 aperture partition arrays with average aperture diameters of 301 ± 5 μm. We addressed the electro-physical properties of the lipid bilayers established across the micro-structured scaffold arrays by controllable reconstitution of biotechnological and physiological relevant membrane peptides and proteins. Next, we tested the scalability of the biomimetic membrane design by establishing lipid bilayers in rectangular 24 x 24 and hexagonal 24 x 27 aperture arrays, respectively. The results presented show that the design is suitable for further developments of sensitive biosensor assays, and furthermore demonstrate that the design can conveniently be scaled up to support planar lipid bilayers in large square-centimeter partition arrays. [graphic removed]