Identification of Pinosylvin in Pinus nigra subsp. laricio : A Naturally Occurring Stilbenoid Suppressing LPS-Induced Expression of Pro-Inflammatory Cytokines and Mediators and Inhibiting the JAK/STAT Signaling Pathway

Stilbenoids, a group of phytoalexin polyphenols produced by plants as a defence mechanism in response to stress conditions, are known for their anti-inflammatory potential. Pinosylvin, a naturally occurring molecule traditionally found in pinus trees, was here identified in subsp. var. from Southern...

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Published inPharmaceuticals (Basel, Switzerland) Vol. 16; no. 5; p. 718
Main Authors Perri, Maria Rosaria, Pellegrino, Michele, Marrelli, Mariangela, Aquaro, Stefano, Cavaliere, Fabiola, Grande, Fedora, Occhiuzzi, Maria Antonietta, Lupia, Carmine, Toma, Claudia-Crina, Conforti, Filomena, Statti, Giancarlo
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 09.05.2023
MDPI
Subjects
Cancer
Cell growth
Cytokines
Growth factors
HPLC
IL-6
Inflammation
molecular docking
Phosphorylation
Phytochemicals
Pinus extract
R&D
RAW 264.7
Research & development
Tumor necrosis factor-TNF
Italy
molecular docking
TNF-α
inflammation
RAW 264.7
resveratrol
HPLC
IL-6
Pinus extract
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Summary:Stilbenoids, a group of phytoalexin polyphenols produced by plants as a defence mechanism in response to stress conditions, are known for their anti-inflammatory potential. Pinosylvin, a naturally occurring molecule traditionally found in pinus trees, was here identified in subsp. var. from Southern Italy through HPLC analysis. Both this molecule and its well-known analogue resveratrol, the most famous wine polyphenol, were compared for their in vitro potential anti-inflammatory activity. Pinosylvin significantly inhibited the release of pro-inflammatory cytokines (TNF-α and IL-6) and NO mediator in LPS-stimulated RAW 264.7 cells. Moreover, its ability to inhibit the JAK/STAT signaling pathway was assessed: Western blot analyses showed a downregulation of both phosphorylated JAK2 and STAT3 proteins. Finally, in order to verify whether this biological activity could be attributed to a direct interaction of pinosylvin with JAK2, a molecular docking study was performed, confirming the capability of pinosylvin to bind the active site of the protein.
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These authors equally contributed to this work.
These authors jointly supervised and equally contributed to this work.
ISSN:1424-8247
1424-8247
DOI:10.3390/ph16050718