Neutralization of Human Papillomavirus Type 11 (HPV-11) by Serum from Women Vaccinated with Yeast-Derived HPV-11 L1 Virus-like Particles: Correlation with Competitive Radioimmunoassay Titer

• Published in: The Journal of infectious diseases Vol. 184; no. 9; pp. 1183 - 1186
• Main Authors: Brown, Darron R., Bryan, Janine T., Schroeder, Jill M., Robinson, Tiffany S., Fife, Kenneth H., Wheeler, Cosette M., Barr, Eliav, Smith, Paula R., Chiacchierini, Lisa, DiCello, Anthony, Jansen, Kathrin U.
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.11.2001; University of Chicago Press
• Subjects: Adolescent; Adult; Animals; Antibodies, Viral - blood; Antibodies, Viral - immunology; Biological and medical sciences; Dose-Response Relationship, Drug; Double-Blind Method; Female; Human papillomavirus 11; Human viral diseases; Humans; Infectious diseases; Medical sciences; Mice; Neutralization Tests; Papillomaviridae - genetics; Papillomaviridae - immunology; Papillomavirus Infections - prevention & control; Papillomavirus Infections - virology; Radioimmunoassay; Tumor Virus Infections - prevention & control; Tumor Virus Infections - virology; Vaccination; Vaccines, Synthetic - immunology; Viral diseases; Viral diseases of the genital and urinary system; Viral diseases of the lymphoid tissue and the blood. Aids; Viral Vaccines - immunology; Virion - immunology; Yeasts - genetics; Human; Yeast; Antibody; Secretion; Use; Vaccination; Rodentia; Vaccine; Papovaviridae; Female genital diseases; Papillomavirus; Virus; Vertebrata; Human papillomavirus; Mammalia; Immunogenicity; Mouse; Radioimmunoassay; Primates; Female; Serum; Cervicitis; Uterine cervix diseases; Neutralization
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/323645

Neutralization of human papillomavirus type 11 (HPV-11) has been demonstrated using serum and cervical secretions from primates vaccinated with virus-like particles (VLPs). Theoretically, neutralizing antibodies could protect women from HPV infection. The immunogenicity of a yeast-derived HPV-11 L1 VLP vaccine was tested in women. Serum specimens were evaluated for HPV-11 titer by competitive radioimmunoassay (cRIA) and for neutralization by use of the athymic mouse xenograft system. Analysis of serum from 104 subjects showed a dose response in HPV-11 cRIA titers and neutralization. Overall, 68 (82.9%) of 82 postimmunization serum specimens from VLP recipients were 100% neutralizing when used in the assay at a 1:50 dilution. Of 69 serum specimens, 63 (91.3%) with cRIA titers >200 milliMerck units per milliliter were neutralizing. Immunization with HPV VLPs elicits a vigorous serum immune response in a high percentage of women. The HPV-11 cRIA titer appears to be a surrogate marker for neutralization