Using the local immune response from the natural buffalo host to generate an antibody fragment library that binds the early larval stages of Schistosoma japonicum

• Published in: International journal for parasitology Vol. 45; no. 11; pp. 729 - 740
• Main Authors: Hosking, Christopher G., Driguez, Patrick, McWilliam, Hamish E.G., Ilag, Leodevico L., Gladman, Simon, Li, Yuesheng, Piedrafita, David, McManus, Donald P., Meeusen, Els N.T., de Veer, Michael J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.09.2015
• Subjects: Animals; Antibodies, Helminth - immunology; Antibodies, Helminth - isolation & purification; Antigens, Helminth - immunology; Buffalo; Buffaloes; China; Larva - growth & development; Larva - immunology; Lymph Nodes - immunology; Phage-display; Protein Array Analysis; Protein microarray; scFv; Schistosoma japonicum; Schistosoma japonicum - growth & development; Schistosoma japonicum - immunology; Schistosomiasis japonica - immunology; Schistosomiasis japonica - veterinary; Schistosomule; Single-Chain Antibodies - immunology; Single-Chain Antibodies - isolation & purification; Single-chain Fv antibody; Single-chain Fv antibody; scFv; Schistosomule; China; Phage-display; Protein microarray; Schistosoma japonicum; Buffalo
• ISSN: 0020-7519; 1879-0135
• DOI: 10.1016/j.ijpara.2015.05.002

[Display omitted] •Use of a natural host (Bubalus bubalis) of Schistosoma japonicum to study antibody–antigen interactions.•Expression of B. bubalis B-cell heavy and light chain variable region (VH, VL) repertoire on the phage particle surface.•Longer single-chain antibody Fv domain (scFv)-associated CDRH3 is more common following selection against S. japonicum.•Creation of an scFv-phage library that specifically binds S. japonicum schistosomules.•Identification of immune exposed proteins using a schistosome-specific microarray. Antibodies isolated from the local draining inguinal lymph node of field exposed-water buffaloes following challenge with Schistosoma japonicum cercariae showed high reactivity towards S. japonicum antigen preparations and bound specifically to formaldehyde-fixed S. japonicum schistosomules. Using this specific local immune response we produced a series of single-chain antibody Fv domain libraries from the same lymph nodes. Removal of phage that cross reacted with epitopes on adult parasites yielded a single-chain antibody Fv domain-phage library that specifically bound to whole formaldehyde-fixed and live S. japonicum schistosomules. DNA sequencing indicated clear enrichment of the single-chain antibody Fv domain library for buffalo B-cell complementarity determining regions post-selection for schistosomule binding. This study also revealed that long heavy chain complementarity determining regions appear to be an important factor when selecting for antibody binding fragments against schistosomule proteins. The selected single-chain antibody Fv domain-phage were used to probe a schistosome-specific protein microarray, which resulted in the recognition of many proteins expressed across all schistosome life-cycle stages. Following absorption to adult worms, the single-chain antibody Fv domain-phage library showed significantly reduced binding to most proteins, whilst two proteins (NCBI GenBank accession numbers AY915878 and AY815196) showed increased binding. We have thus developed a unique set of host derived single-chain antibody Fv domains comprising buffalo B-cell variable regions that specifically bind to early S. japonicum life-stages.