Recyclable magnetite nanoparticle coated with cationic polymers for adsorption of DNA

• Published in: Journal of biomaterials science. Polymer ed. Vol. 27; no. 11; pp. 1200 - 1210
• Main Authors: Rutnakornpituk, B., Theppaleak, T., Rutnakornpituk, M., Vilaivan, T.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 23.07.2016
• Subjects: Adsorption; Cationic; Deoxyribonucleic acid; DNA; DNA - chemistry; Ethers; Humans; ionic adsorption; Magnetite; Magnetite Nanoparticles - chemistry; Methacrylates - chemistry; nanoparticle; Nanostructure; Particle Size; Polyethylene Glycols - chemistry; Polymerization; Polymers; recyclable; Surface chemistry; Surface Properties; nanoparticle; ionic adsorption; Magnetite; recyclable; DNA
• ISSN: 0920-5063; 1568-5624
• DOI: 10.1080/09205063.2016.1189378

Magnetite nanoparticle (MNP) grafted with a cationic copolymer between poly(2-(N,N-diethylamino) ethyl methacrylate) and poly(poly(ethylene glycol) methyl ether methacrylate)) for efficient and recyclable adsorption of 5'-fluorescein-tagged DNA (FAM-dT 9 ) was prepared. MNP having highest degree of positive charge (+32.1 ± 1.9 mV) retained 100% adsorption of FAM-dT 9 during eight adsorption-separation-desorption cycles. The MNP having lower degree of positive charge showed a slight decrease in adsorption percentages (94-98% adsorption) after multiple recycling processes. This biocompatible hybrid material with charged surface and magnetic-responsive properties might be applicable for use as a nanosolid support for efficient and facile separation of various bioentities.